Unraveling amyloid formation paths of Parkinson's disease protein alpha-synuclein triggered by anionic vesicles

• Amyloid formation of the synaptic brain protein alpha-synuclein (alpha S) is related to degeneration of dopaminergic neurons in Parkinson's disease patients. aS is thought to function in vesicle transport and fusion and it binds strongly to negatively charged vesicles in vitro. Here we combined circular dichroism, fluorescence and imaging methods in vitro to characterize the interaction of alpha S with negatively charged vesicles of DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine, sodium salt) and DOPG (1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol), sodium salt) and the consequences of such interactions on alpha S amyloid formation. We found that lipid head-group chemistry modulates alpha S interactions and also affects amyloid fiber formation. During the course of the experiments, we made the unexpected discovery that pre-formed alpha S oligomers, typically present in a small amount in the alpha S starting material, acted as templates for linear growth of anomalous amyloid fibers in the presence of vesicles. At the same time, the remaining alpha S monomers were restricted from vesicle-mediated nucleation of amyloid fibers. Although not a dominant process in bulk experiments, this hidden alpha S aggregation pathway may be of importance in vivo.

Subject headings

NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)

Keyword

mutation

fibrillation

fibrillogenesis

aggregation

membrane interactions

packing defects

lipid vesicles

phospholipid-binding

states

solution nmr-spectroscopy

Publication and Content Type

art (subject category)

ref (subject category)