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Maintenance of neur...
Maintenance of neural progenitor cell stemness in 3D hydrogels requires matrix remodelling
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- Madl, Christopher M. (author)
- Stanford University
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- Lesavage, Bauer L. (author)
- Stanford University
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- Dewi, Ruby E. (author)
- Stanford University
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- Dinh, Cong B. (author)
- Stanford University
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- Stowers, Ryan S. (author)
- Stanford University
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- Khariton, Margarita (author)
- Stanford University
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- Lampe, Kyle J. (author)
- University of Virginia,Stanford University
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- Nguyen, Thuy Duong, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Chaudhuri, Ovijit (author)
- Stanford University
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- Enejder, Annika, 1969 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Heilshorn, S. C. (author)
- Stanford University
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(creator_code:org_t)
- 2017-10-30
- 2017
- English.
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In: Nature Materials. - : Springer Science and Business Media LLC. - 1476-4660 .- 1476-1122. ; 16:12, s. 1233-1242
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Abstract
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- Neural progenitor cell (NPC) culture within three-dimensional (3D) hydrogels is an attractive strategy for expanding a therapeutically relevant number of stem cells. However, relatively little is known about how 3D material properties such as stiffness and degradability affect the maintenance of NPC stemness in the absence of differentiation factors. Over a physiologically relevant range of stiffness from â 1/40.5 to 50 kPa, stemness maintenance did not correlate with initial hydrogel stiffness. In contrast, hydrogel degradation was both correlated with, and necessary for, maintenance of NPC stemness. This requirement for degradation was independent of cytoskeletal tension generation and presentation of engineered adhesive ligands, instead relying on matrix remodelling to facilitate cadherin-mediated cell-cell contact and promote ?-catenin signalling. In two additional hydrogel systems, permitting NPC-mediated matrix remodelling proved to be a generalizable strategy for stemness maintenance in 3D. Our findings have identified matrix remodelling, in the absence of cytoskeletal tension generation, as a previously unknown strategy to maintain stemness in 3D.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Madl, Christophe ...
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Lesavage, Bauer ...
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Dewi, Ruby E.
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Dinh, Cong B.
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Stowers, Ryan S.
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Khariton, Margar ...
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show more...
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Lampe, Kyle J.
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Nguyen, Thuy Duo ...
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Chaudhuri, Oviji ...
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Enejder, Annika, ...
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Heilshorn, S. C.
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show less...
- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
- Articles in the publication
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Nature Materials
- By the university
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Chalmers University of Technology