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Saccharomyces cerev...
Saccharomyces cerevisiae displays a stable transcription start site landscape in multiple conditions
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- Börlin, Christoph Sebastian, 1989 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Cvetesic, Nevena (author)
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- Holland, Petter, 1985 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bergenholm, David, 1987 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Siewers, Verena, 1976 (author)
- Novo Nordisk Fonden,Novo Nordisk Foundation,Chalmers tekniska högskola,Chalmers University of Technology
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- Lenhard, Boris (author)
- Universitetet i Bergen,University of Bergen
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- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Novo Nordisk Fonden,Novo Nordisk Foundation,Danmarks Tekniske Universitet,Technical University of Denmark
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(creator_code:org_t)
- 2018-12-22
- 2019
- English.
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In: FEMS Yeast Research. - : Oxford University Press (OUP). - 1567-1356 .- 1567-1364. ; 19:2
- Related links:
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https://academic.oup...
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https://doi.org/10.1...
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Abstract
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- One of the fundamental processes that determine cellular fate is regulation of gene transcription. Understanding these regulatory processes is therefore essential for understanding cellular responses to changes in environmental conditions. At the core promoter, the regulatory region containing the transcription start site (TSS), all inputs regulating transcription are integrated. Here, we used Cap Analysis of Gene Expression (CAGE) to analyze the pattern of TSSs at four different environmental conditions (limited in ethanol, limited in nitrogen, limited in glucose and limited in glucose under anaerobic conditions) using the Saccharomyces cerevisiae strain CEN.PK113-7D. With this experimental setup, we were able to show that the TSS landscape in yeast is stable at different metabolic states of the cell. We also show that the spatial distribution of transcription initiation events, described by the shape index, has a surprisingly strong negative correlation with measured gene expression levels, meaning that genes with higher expression levels tend to have a broader distribution of TSSs. Our analysis supplies a set of high-quality TSS annotations useful for metabolic engineering and synthetic biology approaches in the industrially relevant laboratory strain CEN.PK113-7D, and provides novel insights into yeast TSS dynamics and gene regulation.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
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- art (subject category)
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- By the author/editor
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Börlin, Christop ...
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Cvetesic, Nevena
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Holland, Petter, ...
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Bergenholm, Davi ...
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Siewers, Verena, ...
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Lenhard, Boris
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show more...
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Nielsen, Jens B, ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Genetics
- Articles in the publication
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FEMS Yeast Resea ...
- By the university
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Chalmers University of Technology