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Dynamic Range Expan...
Dynamic Range Expansion of the C-Reactive Protein Quantification with a Tandem Giant Magnetoresistance Biosensor
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- Meng, Fanda, 1987 (author)
- Shandong Qianfoshan Hospital,Chalmers tekniska högskola,Chalmers University of Technology
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- Zhang, Lei (author)
- Chinese Academy of Sciences,Dongguan Bosh Biotechnologies, Ltd.
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- Huo, Weisong (author)
- Dongguan Bosh Biotechnologies, Ltd.
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- Lian, Jie (author)
- People's Public Security University of China
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- Jesorka, Aldo, 1967 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Shi, Xizeng (author)
- Dongguan Bosh Biotechnologies, Ltd.
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- Gao, Yunhua (author)
- Chinese Academy of Sciences
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(creator_code:org_t)
- 2021-05-07
- 2021
- English.
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In: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 6:19, s. 12923-12930
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Abstract
Subject headings
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- In this study, we report a convenient analytical method for a full-range quantification of the C-reactive protein (CRP), a blood biomarker of infection and cardiovascular events. We determine CRP over the entire diagnostically relevant concentration range in undiluted human blood serum in a single test, using a tandem giant magnetoresistance (GMR) sensor. The tandem principle combines a sandwich assay and a competitive assay, which allows for the discrimination of the concentration values resulting from the multivalued dose-response curve ("Hook"effect), which characterizes the one-step sandwich assay at high CRP concentrations. The sensor covers a linear detection range for CRP concentration from 3 ng/mL to 350 μg/mL, the detection limit (s/n = 3) is 1 ng/mL. The prominent features of the chip-based method are its expanded dynamic range and low sample volume (50 μL), and the need for a short measurement time of 15 min. These figures of merit, in addition to the low detection limit equal to the established assay instrumentation, make it a viable candidate for use in point-of-care diagnostics.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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