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Encapsulation-Induc...
Encapsulation-Induced Stress Helps Saccharomyces cerevisiae Resist Convertible Lignocellulose Derived Inhibitors
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- Westman, Johan, 1983 (author)
- Högskolan i Borås,Institutionen Ingenjörshögskolan,Biotechnology
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- Manikondu, R. B. (author)
- Högskolan i Borås,University of Borås
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- Franzén, Carl Johan, 1966 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Taherzadeh Esfahani, Mohammad, 1965 (author)
- Högskolan i Borås,University of Borås
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- Taherzadeh, Mohammad J (author)
- Högskolan i Borås,Institutionen Ingenjörshögskolan,Biotechnology
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(creator_code:org_t)
- 2012-09-19
- 2012
- English.
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In: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 13:9, s. 11881-11894
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Abstract
Subject headings
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- The ability of macroencapsulated Saccharomyces cerevisiae CBS8066 to withstand readily and not readily in situ convertible lignocellulose-derived inhibitors was investigated in anaerobic batch cultivations. It was shown that encapsulation increased the tolerance against readily convertible furan aldehyde inhibitors and to dilute acid spruce hydrolysate, but not to organic acid inhibitors that cannot be metabolized anaerobically. Gene expression analysis showed that the protective effect arising from the encapsulation is evident also on the transcriptome level, as the expression of the stress-related genes YAP1, ATR1 and FLR1 was induced upon encapsulation. The transcript levels were increased due to encapsulation already in the medium without added inhibitors, indicating that the cells sensed low stress level arising from the encapsulation itself. We present a model, where the stress response is induced by nutrient limitation, that this helps the cells to cope with the increased stress added by a toxic medium, and that superficial cells in the capsules degrade convertible inhibitors, alleviating the inhibition for the cells deeper in the capsule.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Keyword
- anaerobic batch fermentation
- furfural
- acid
- pretreatment
- microencapsulation
- lignocellulosic hydrolysate
- conversion
- ethanol
- carboxylic acids
- HMF (5-hydroxymethyl furfural)
- glucose
- ethanol-production
- yeast-cells
- hydrolyzate
- continuous cultivation
- Resursåtervinning
Publication and Content Type
- art (subject category)
- ref (subject category)
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