TARGETING THE INTRACELLULAR REDOX STATE IN THE DEVELOPMENT OF MORE ROBUST Saccharomyces cerevisiae STRAINS FOR LIGNOCELLULOSIC BIOETHANOL PRODUCTION

Ask, Magnus, 1983 (author): Chalmers tekniska högskola,Chalmers University of Technology

Bettiga, Maurizio, 1978 (author): Chalmers tekniska högskola,Chalmers University of Technology

Mapelli, Valeria, 1978 (author): Chalmers tekniska högskola,Chalmers University of Technology

Raju Duraiswamy, Varuni, 1987 (author): Chalmers tekniska högskola,Chalmers University of Technology

Höck, Heidi (author): Chalmers tekniska högskola,Chalmers University of Technology

Olsson, Lisbeth, 1963 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

Abstract Subject headings

Bioethanol produced from lignocellulosic raw materials is a promising alternative to fossil fuels and to decrease greenhouse gas emissions, but several challenges still exist. When lignocellulosic biomass is pretreated, a number of undesired degradation products are generated, among which the furaldehydes furfural and hydroxymethylfurfural (HMF) have shown to impede growth and limit ethanol productivity of the yeast Saccharomyces cerevisiae. In the present study, a recombinant, xylose-utilizing S. cerevisiae strain was challenged with sub-lethal concentrations of furfural and HMF in anaerobic batch cultivations. By pulsing furaldehydes in either the glucose or the xylose consumption phase, perturbations in the intracellular NAD(P)H/NAD(P)+ ratios could be demonstrated. A genome-wide study of transcription found that genes related to NADPH-requiring processes, such as nitrogen and sulphur assimilation, were significantly induced. Moreover, the protective metabolite and antioxidant glutathione was identified as the highest scoring reporter metabolite in the transcriptome analysis. S. cerevisiae strains overproducing glutathione were constructed and the resulting strains were evaluated in simultaneous saccharification and fermentation (SSF) of pretreated spruce. The results from the present study provide valuable insights of how S. cerevisiae responds to stress imposed by HMF and furfural and how such information could be used to engineer more robust yeast strains.

By the author/editor: Ask, Magnus, 198 ...; Bettiga, Maurizi ...; Mapelli, Valeria ...; Raju Duraiswamy, ...; Höck, Heidi; Olsson, Lisbeth, ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bioenergy

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