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Ferritin-supported ...
Ferritin-supported lipid bilayers for triggering the endothelial cell response
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- Satriano, C. (author)
- Universita degli Studi di Catania,University of Catania
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- Lupo, G. (author)
- Universita degli Studi di Catania,University of Catania
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- Motta, C. (author)
- Universita degli Studi di Catania,University of Catania
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- Anfuso, C. D. (author)
- Universita degli Studi di Catania,University of Catania
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- Di Pietro, P. (author)
- Universita degli Studi di Catania,University of Catania
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- Kasemo, Bengt Herbert, 1942 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
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In: Colloids and Surfaces B: Biointerfaces. - : Elsevier BV. - 0927-7765 .- 1873-4367. ; 149, s. 48-55
- Related links:
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http://dx.doi.org/10...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Hybrid nanoassemblies of ferritin and silica-supported lipid bilayers (ferritin-SLBs) have been prepared and tested for the adhesion, spreading and proliferation of retinal microvascular endothelial cells (ECs). Lipid membranes with varying surface charge were obtained by mixing cationic 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (POEPC) with zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) at increasing POPC/POEPC ratios. The supported bilayer formation and their subsequent interaction processes with ferritin were studied at the pH of 7.4 at different protein concentrations, by using the quartz crystal microbalance with dissipation monitoring and by atomic force microscopy. Both kinetics and viscoelastic parameters of the protein-lipid membrane interface were scrutinized, as well as surface coverage. Phase-contrast optical microscopy analyses of the ferritin-SLBs substrates after their interaction with endothelial cells evidenced the highest cell adhesion (2-4 h of incubation time) and proliferation (from 24 h to 5 days) for the membranes of POPC/POEPC (75:25 ratio). Moreover, ferritin increased both cell adhesion and proliferation in comparison to control glass (respectively 1.5- and 1.75-fold) as well as proliferation in comparison to bare POPC/POEPC (95:5 ratio) (2 fold). Results are very promising in the goal of modulating the endothelial cell response through the interplay of viscoelastic/charge properties of the solid-supported membranes and the SLB-conditioned ferritin activity.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Protein-lipid membrane nanoassembly
- QCM-D
- Viscoelastic properties
- AFM
- Silica supported lipid membranes
- Retinal microvascular endothelial cells
Publication and Content Type
- art (subject category)
- ref (subject category)
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