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Generation of focused electric field patterns at dielectric surfaces

Olofsson, Jessica, 1975 (author)
Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
Levin, M. (author)
Cellectricon AB, Sweden
Stromberg, A. (author)
Cellectricon AB, Sweden,Ytbioteknik
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Weber, S. G. (author)
University of Pittsburgh,University of Pittsburgh, USA
Ryttsen, F. (author)
Cellectricon AB, Sweden
Orwar, Owe, 1964 (author)
Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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 (creator_code:org_t)
2005-06-18
2005
English.
In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 77:14, s. 4667-4672
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • We here report on a concept for creating well-defined electric field gradients between the boundaries of capillary electrode (a capillary of a nonconducting material equipped with an interior metal electrode) outlets, and dielectric surfaces. By keeping a capillary electrode opening close to a boundary between a conducting solution and a nonconducting medium, a high electric field can be created close to the interface by field focusing effects. By varying the inner and outer diameters of the capillary, the span of electric field strengths and the field gradient obtained can be controlled, and by varying the slit height between the capillary rim and the surface, or the applied current, the average field strength and gradient can be varied. Field focusing effects and generation of electric field patterns were analyzed using finite element method simulations. We experimentally verified the method by electroporation of a fluorescent dye (fluorescein diphosphate) into adherent, monolayered cells (PC-12 and WSS-1) and obtained a pattern of fluorescent cells corresponding to the focused electric field.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Kemiteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Chemical Engineering (hsv//eng)
NATURVETENSKAP  -- Kemi -- Materialkemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Materials Chemistry (hsv//eng)

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art (subject category)
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