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Lignin boosts the c...
Lignin boosts the cellulase performance of a GH-61 enzyme from Sporotrichum thermophile
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- Dimarogona, M. (author)
- National Technical University of Athens (NTUA),Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens
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- Topakas, E. (author)
- National Technical University of Athens (NTUA),Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens
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- Olsson, Lisbeth, 1963 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Christakopoulos, Paul (author)
- National Technical University of Athens (NTUA)
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
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In: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 110, s. 480-487
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https://doi.org/10.1...
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Abstract
Subject headings
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- An enzyme belonging to the glycoside hydrolase family 61 from the thermophilic fungus Sporotrichum thermophile, was functionally expressed in the methylotrophic yeast Pichia pastoris under the transcriptional control of the alcohol oxidase (AOX1) promoter. The enzyme hydrolyzed barley beta-glucan, carboxymethyl cellulose, lichenan, wheat arabinoxylan and birchwood xylan showing optimal activity at pH 8 and 65 degrees C. A 2:1 mixture of Celluclast 1.5 L and StCel61a was capable of increasing the degree of spruce conversion by 42%. The use of substrates with varying lignin content permitted the detection of a dependence of the enhancing capacity of StCel61a on the radical scavenging capacity of the different lignocellulosics. In the presence of a reductant, StCel61a boosted the efficiency of a mixture of purified cellulases (EGII, CBHI, beta-GLUC) by 20%. The synergistic activity exhibited by StCel61a and its dependence on reducing substances provide guidelines for process design towards the production of economically viable bioethanol.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Naturresursteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Engineering (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Keyword
- degradation
- treated wheat straw
- Cellulose hydrolysis
- endoglucanase
- trichoderma-reesei
- Glycoside hydrolase family 61
- growth
- iv
- glycoside hydrolase family
- Spruce-derived material
- expression
- Hydrothermally
- Sporotrichum thermophile
- antioxidant activity
Publication and Content Type
- art (subject category)
- ref (subject category)
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