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Carbon-Ring Microel...
Carbon-Ring Microelectrode Arrays for Electrochemical Imaging of Single Cell Exocytosis: Fabrication and Characterization
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- Lin, Y. Q. (author)
- Göteborgs universitet,University of Gothenburg
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- Trouillon, R. (author)
- Göteborgs universitet,University of Gothenburg
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- Svensson, M. I. (author)
- Göteborgs universitet,University of Gothenburg
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- Keighron, Jacqueline, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Cans, Ann-Sofie, 1971 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ewing, Andrew, 1957 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2012-03-06
- 2012
- English.
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In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 84:6, s. 2949-2954
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Abstract
Subject headings
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- Fabrication of carbon microelectrode arrays, with up to 15 electrodes in total tips as small as 10-50 mu m, is presented. The support structures of microelectrodes were obtained by pulling multiple quartz capillaries together to form hollow capillary arrays before carbon deposition. Carbon ring microelectrodes were deposited by pyrolysis of acetylene in the lumen of these quartz capillary arrays. Each carbon deposited array tip was filled with epoxy, followed by beveling of the tip of the array to form a deposited carbon-ring microelectrode array (CRMA). Both the number of the microelectrodes in the array and the tip size are independently tunable. These CRMAs have been characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, and electrogenerated chemiluminescence. Additionally, the electrochemical properties were investigated with steady-state voltammetry. In order to demonstrate the utility of these fabricated microelectrodes in neurochemistry, CRMAs containing eight microring electrodes were used for electrochemical monitoring of exocytotic events from single PC12 cells. Subcellular temporal heterogeneities in exocytosis (i.e. cold spots vs hot spots) were successfully detected with the CRMAs.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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