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Protein kinase A co...
Protein kinase A controls yeast growth in visible light
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- Molin, Mikael, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Logg, Katarina, 1979 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Bodvard, Kristofer, 1981 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Peeters, Ken, 1985 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Forsmark, Annabelle, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Roger, Friederike (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Jorhov, Anna (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Mishra, Neha (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Billod, Jean Marc (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Amir, Sabiha (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Andersson, Mikael (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Eriksson, Leif A, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Warringer, Jonas, 1973 (author)
- Gothenburg University,Göteborgs universitet,CARe - Centrum för antibiotikaresistensforskning,Institutionen för kemi och molekylärbiologi,Linnécentrum för marin evolutionsbiologi (CEMEB),Centre for antibiotic resistance research, CARe,Department of Chemistry and Molecular Biology,Linnaeus Centre for Marine Evolutionary Biology (CEMEB)
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- Käll, Mikael, 1963 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Blomberg, Anders, 1956 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Institutionen för kemi och molekylärbiologi,Linnécentrum för marin evolutionsbiologi (CEMEB),Department of marine sciences,Department of Chemistry and Molecular Biology,Linnaeus Centre for Marine Evolutionary Biology (CEMEB)
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(creator_code:org_t)
- 2020-11-16
- 2020
- English.
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In: BMC Biology. - : Springer Science and Business Media LLC. - 1741-7007. ; 18:1
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Abstract
Subject headings
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- Background: A wide variety of photosynthetic and non-photosynthetic species sense and respond to light, having developed protective mechanisms to adapt to damaging effects on DNA and proteins. While the biology of UV light-induced damage has been well studied, cellular responses to stress from visible light (400–700 nm) remain poorly understood despite being a regular part of the life cycle of many organisms. Here, we developed a high-throughput method for measuring growth under visible light stress and used it to screen for light sensitivity in the yeast gene deletion collection. Results: We found genes involved in HOG pathway signaling, RNA polymerase II transcription, translation, diphthamide modifications of the translational elongation factor eEF2, and the oxidative stress response to be required for light resistance. Reduced nuclear localization of the transcription factor Msn2 and lower glycogen accumulation indicated higher protein kinase A (cAMP-dependent protein kinase, PKA) activity in many light-sensitive gene deletion strains. We therefore used an ectopic fluorescent PKA reporter and mutants with constitutively altered PKA activity to show that repression of PKA is essential for resistance to visible light. Conclusion: We conclude that yeast photobiology is multifaceted and that protein kinase A plays a key role in the ability of cells to grow upon visible light exposure. We propose that visible light impacts on the biology and evolution of many non-photosynthetic organisms and have practical implications for how organisms are studied in the laboratory, with or without illumination.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
Keyword
- Light sensitivity
- HOG signaling
- Genome-wide screen
- Protein kinase A
- Yeast
- Diphthamide modification
- Visible light
Publication and Content Type
- art (subject category)
- ref (subject category)
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Molin, Mikael, 1 ...
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Logg, Katarina, ...
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Bodvard, Kristof ...
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Peeters, Ken, 19 ...
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Forsmark, Annabe ...
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Roger, Friederik ...
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Jorhov, Anna
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Mishra, Neha
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Billod, Jean Mar ...
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Amir, Sabiha
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Andersson, Mikae ...
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Eriksson, Leif A ...
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Warringer, Jonas ...
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Käll, Mikael, 19 ...
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Blomberg, Anders ...
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BMC Biology
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Chalmers University of Technology
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University of Gothenburg