SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:research.chalmers.se:f0a87737-a7f8-47a4-bb6f-971afbf8e638"
 

Search: onr:"swepub:oai:research.chalmers.se:f0a87737-a7f8-47a4-bb6f-971afbf8e638" > Mismatch detection ...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Nordén, Bengt,1945Chalmers tekniska högskola,Chalmers University of Technology (author)

Mismatch detection in homologous strand exchange amplified by hydrophobic effects

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • 2021-03-29
  • Wiley,2021
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:research.chalmers.se:f0a87737-a7f8-47a4-bb6f-971afbf8e638
  • https://doi.org/10.1002/bip.23426DOI
  • https://research.chalmers.se/publication/523403URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • In contrast to DNA replication and transcription where nucleotides are added and matched one by one, homologous recombination by DNA strand exchange tests whole sequences for complementarity, which requires elimination of mismatched yet thermodynamically stable intermediates. To understand the remarkable sequence specificity of homologous recombination, we have studied strand exchange between a 20-mer duplex containing one single mismatch (placed at varied positions) with the matching single strand in presence of poly(ethylene glycol) representing a semi-hydrophobic environment. A FRET-based assay shows that rates and yields of strand exchange from mismatched to matched strands rapidly increase with semi-hydrophobic co-solute concentration, contrasting previously observed general strand exchange accelerating effect of ethyl glycol ethers. We argue that this effect is not caused simply by DNA melting or solvent-induced changes of DNA conformation but is more complex involving several mechanisms. The catalytic effects, we propose, involve strand invasion facilitated by reduced duplex stability due to weakened base stacking (“longitudinal breathing”). Secondly, decreased water activity makes base-pair hydrogen bonds stronger, increasing the relative energy penalty per mismatch. Finally, unstacked mismatched bases (gaps) are stabilized through partly intercalated hydrophobic co-solvent molecules, assisting nucleation of strand invasion at the point of mismatch. We speculate that nature long ago discovered, and now exploits in various enzymes, that sequence recognition power of nucleic acids may be modulated in a hydrophobic environment.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Brown, T.University Of Oxford (author)
  • Feng, Bobo,1987Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)bobo (author)
  • Chalmers tekniska högskolaUniversity Of Oxford (creator_code:org_t)

Related titles

  • In:Biopolymers: Wiley112:40006-35251097-0282

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Find more in SwePub

By the author/editor
Nordén, Bengt, 1 ...
Brown, T.
Feng, Bobo, 1987
About the subject
NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
and Biochemistry and ...
NATURAL SCIENCES
NATURAL SCIENCES
and Biological Scien ...
and Microbiology
ENGINEERING AND TECHNOLOGY
ENGINEERING AND ...
and Industrial Biote ...
and Other Industrial ...
Articles in the publication
Biopolymers
By the university
Chalmers University of Technology

Search outside SwePub

Wikipedia about the author:
Bengt_Nordén
Nordén, Bengt,1945
en

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view