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Increased CO 2 fixa...
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Qin, NingBeijing University of Chemical Technology
(author)
Increased CO 2 fixation enables high carbon-yield production of 3-hydroxypropionic acid in yeast
- Article/chapterEnglish2024
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2024
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LIBRIS-ID:oai:research.chalmers.se:f1d9db90-02f7-489c-8c91-10f6c67f92d5
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https://doi.org/10.1038/s41467-024-45557-9DOI
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https://research.chalmers.se/publication/540106URI
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
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CO2 fixation plays a key role to make biobased production cost competitive. Here, we use 3-hydroxypropionic acid (3-HP) to showcase how CO2 fixation enables approaching theoretical-yield production. Using genome-scale metabolic models to calculate the production envelope, we demonstrate that the provision of bicarbonate, formed from CO2, restricts previous attempts for high yield production of 3-HP. We thus develop multiple strategies for bicarbonate uptake, including the identification of Sul1 as a potential bicarbonate transporter, domain swapping of malonyl-CoA reductase, identification of Esbp6 as a potential 3-HP exporter, and deletion of Uga1 to prevent 3-HP degradation. The combined rational engineering increases 3-HP production from 0.14 g/L to 11.25 g/L in shake flask using 20 g/L glucose, approaching the maximum theoretical yield with concurrent biomass formation. The engineered yeast forms the basis for commercialization of bio-acrylic acid, while our CO2 fixation strategies pave the way for CO2 being used as the sole carbon source.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Li, LingyunBeijing University of Chemical Technology
(author)
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Wan, XiaozhenBeijing University of Chemical Technology
(author)
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Ji, XuBeijing University of Chemical Technology
(author)
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Chen, Yu,1990Shenzhen Institute of Advanced Technology(Swepub:cth)cheyu
(author)
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Li, ChaokunHelsingin Yliopisto,University of Helsinki
(author)
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Liu, PingBeijing University of Chemical Technology
(author)
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Zhang, YijieBeijing University of Chemical Technology
(author)
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Yang, WeijieBeijing University of Chemical Technology
(author)
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Jiang, Junfeng
(author)
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Xia, Jianye
(author)
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Shi, Shuobo,1981Beijing University of Chemical Technology(Swepub:cth)shuobos
(author)
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Tan, TianweiBeijing University of Chemical Technology
(author)
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Nielsen, Jens B,1962Chalmers tekniska högskola,Chalmers University of Technology,BioInnovation Institute (BII),Beijing University of Chemical Technology(Swepub:cth)nielsenj
(author)
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Chen, Yun,1978Novo Nordisk Fonden,Novo Nordisk Foundation,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)yunc
(author)
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Liu, Zihe,1984Beijing University of Chemical Technology(Swepub:cth)zihe
(author)
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Beijing University of Chemical TechnologyShenzhen Institute of Advanced Technology
(creator_code:org_t)
Related titles
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In:Nature Communications15:12041-17232041-1723
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- By the author/editor
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Qin, Ning
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Li, Lingyun
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Wan, Xiaozhen
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Ji, Xu
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Chen, Yu, 1990
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Li, Chaokun
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show more...
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Liu, Ping
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Zhang, Yijie
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Yang, Weijie
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Jiang, Junfeng
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Xia, Jianye
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Shi, Shuobo, 198 ...
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Tan, Tianwei
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Nielsen, Jens B, ...
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Chen, Yun, 1978
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Liu, Zihe, 1984
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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Chalmers University of Technology