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Tomato protoplasts ...
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Andersson, MarietteSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding
(author)
Tomato protoplasts as cell target for ribonucleoprotein (RNP)-mediated multiplexed genome editing
- Article/chapterEnglish2021
Publisher, publication year, extent ...
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2020-10-24
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Springer Science and Business Media LLC,2021
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Springer Verlag (Germany),2024
Numbers
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LIBRIS-ID:oai:slubar.slu.se:108725
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https://res.slu.se/id/publ/108725URI
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https://doi.org/10.1007/s11240-020-01954-8DOI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The possibility to produce plants edited in multiple genes by means of DNA-free approaches opens new perspectives for breeding purposes and acceptance of resultant genotypes. In this work, we have explored the polyethylene glycol (PEG)-mediated delivery of ribonucleoproteins (RNPs) in tomato protoplasts using a multiplexing approach (i.e. two genes targeted simultaneously using two sgRNAs per gene) for the first time. We have analysed the editing outcome in fully developed green calli and demonstrated that tomato protoplasts are a valid cell target for RNP-mediated multiplexed genome editing with high efficiency.Key message RNP could be applied with high efficiency in a multiplexing genome editing approach in tomato protoplasts.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Hofvander, PerSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding(Swepub:slu)48735
(author)
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Sveriges lantbruksuniversitetInstitutionen för växtförädling
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Related titles
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In:Plant Cell, Tissue and Organ Culture: Springer Science and Business Media LLC144, s. 463-4670167-68571573-5044
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