Combining transcriptomics and genetic linkage based information to identify candidate genes associated with Heterobasidion-resistance in Norway spruce

Chaudhary, RajivSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology (author)

2020-07-29
Springer Science and Business Media LLC,2020
Nature Publishing Group: Open Access Journals - Option C / Nature Publishing Group,2024

The Heterobasidion annosum s.l species complex comprises the most damaging forest pathogens to Norway spruce. We revisited previously identified Quantitative Trait Loci (QTLs) related to Heterobasidion-resistance in Norway spruce to identify candidate genes associated with these QTLs. We identified 329 candidate genes associated with the resistance QTLs using a gene-based composite map for Pinaceae. To evaluate the transcriptional responses of these candidate genes to H. parviporum, we inoculated Norway spruce plants and sequenced the transcriptome of the interaction at 3 and 7 days post inoculation. Out of 298 expressed candidate genes 124 were differentially expressed between inoculation and wounding control treatment. Interestingly, PaNAC04 and two of its paralogs in the subgroup III-3 of the NAC family transcription factors were found to be associated with one of the QTLs and was also highly induced in response to H. parviporum. These genes are possibly involved in the regulation of biosynthesis of flavonoid compounds. Furthermore, several of the differentially expressed candidate genes were associated with the phenylpropanoid pathway including a phenylalanine ammonia-lyase, a cinnamoyl-CoA reductase, a caffeoyl-CoA O-methyltransferase and a PgMYB11-like transcription factor gene. Combining transcriptome and genetic linkage analyses can help identifying candidate genes for functional studies and molecular breeding in non-model species.

Lundén, KarlSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology(Swepub:slu)50910 (author)
Dalman, KerstinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences(Swepub:slu)46883 (author)
Dubey, MukeshSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology(Swepub:slu)49006 (author)
Nemesio Gorriz, MiguelSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology,Teagasc(Swepub:slu)50185 (author)
Stenlid, JanSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology(Swepub:slu)46689 (author)
Elfstrand, MalinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology(Swepub:slu)48669 (author)
Sveriges lantbruksuniversitetDepartment of Forest Mycology and Plant Pathology (creator_code:org_t)
Sveriges lantbruksuniversitet

By the author/editor: Chaudhary, Rajiv; Lundén, Karl; Dalman, Kerstin; Dubey, Mukesh; Nemesio Gorriz, ...; Stenlid, Jan; show more...; Elfstrand, Malin; show less...

About the subject

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agricultural Bio ...; and Genetics and Bre ...

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agricultural Bio ...; and Plant Biotechnol ...

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