Morphology and Composition of the Spider Major Ampullate Gland and Dragline Silk

Andersson, MarleneSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB) (author)

Spider silk is made of unique proteins-spidroins-secreted and stored as a protein solution (dope) in specialized glands. The major ampullate gland, source of the dragline silk, is composed of a tail, a sac and an elongated duct. For this gland, several different types of epithelial cells and granules have been described, but it is largely unknown how they correlate with spidroin production. It is also not settled what parts of the large spidroins end up in the final silk, and it has been suggested that the N-terminal domain (NT) is lacking. Here we show that NT is present in the dope and throughout drag,line silk fibers, including the skin layer, and that the major ampullate tail and sac consist of three different and sharply demarcated zones (A-C), each with a distinct epithelial cell type. Finally, we show that spidroins are produced in the A and B zone epithelia, while the C zone granules lack spidroins.

Holm, LenaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB)(Swepub:slu)48426 (author)
Ridderstråle, YvonneSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB)(Swepub:slu)51077 (author)
Johansson, JanKarolinska Institutet,Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Karolinska Institute,Tallinn University(Swepub:slu)47966 (author)
Rising, AnnaKarolinska Institutet,Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Karolinska Institute(Swepub:slu)47422 (author)
Sveriges lantbruksuniversitetInstitutionen för anatomi, fysiologi och biokemi (creator_code:org_t)
Sveriges lantbruksuniversitet

In:Biomacromolecules: American Chemical Society (ACS)14, s. 2945-29521525-77971526-4602

By the author/editor: Andersson, Marle ...; Holm, Lena; Ridderstråle, Yv ...; Johansson, Jan; Rising, Anna

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Other Biological ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Medical Biotechn ...

By the university: Swedish University of Agricultural Sciences; Karolinska Institutet

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