Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems

Bödeker, Inga (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig mykologi och patologi,Institutionen för sydsvensk skogsvetenskap,Department of Forest Mycology and Pathology,Southern Swedish Forest Research Centre

Engelbrecht Clemmensen, Karina (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig mykologi och patologi,Department of Forest Mycology and Pathology

Olson, Åke (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology

Lindahl, Björn (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig mykologi och patologi,Department of Forest Mycology and Pathology

(creator_code:org_t)

2014-04-14
2014
English.
In: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 203, s. 245-256

Related links:: https://res.slu.se/i...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypotheses that ectomycorrhizal Cortinarius species produce Mn-dependent peroxidases, and that the activity of these enzymes declines at elevated concentrations of inorganic N.In a boreal pine forest and a sub-arctic birch forest, Cortinarius DNA was assessed by 454-sequencing of ITS amplicons and related to Mn-peroxidase activity in humus samples with- and without previous N amendment. Transcription of Cortinarius Mn-peroxidase genes was investigated in field samples. Phylogenetic analyses of Cortinarius peroxidase amplicons and genome sequences were performed.We found a significant co-localization of high peroxidase activity and DNA from Cortinarius species. Peroxidase activity was reduced by high ammonium concentrations. Amplification of mRNA sequences indicated transcription of Cortinarius Mn-peroxidase genes under field conditions. The Cortinarius glaucopus genome encodes 11 peroxidases - a number comparable to many white-rot wood decomposers.These results support the hypothesis that some ectomycorrhizal fungi - Cortinarius species in particular - may play an important role in decomposition of complex organic matter, linked to their mobilization of organically bound N.

By the author/editor: Bödeker, Inga; Engelbrecht Clem ...; Olson, Åke; Lindahl, Björn

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

AGRICULTURAL SCIENCES: AGRICULTURAL SCI ...; and Agriculture Fore ...; and Forest Science

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