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Development of a ge...
Development of a genetic transformation method for seabuckthorn ( Hippophae rhamnoides L.).
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- Sriskandarajah, Sridevy (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
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- Clapham, David (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
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- Lundquist, Per-Olof (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
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(creator_code:org_t)
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- Scientific Research Publishing, Inc. 2014
- 2014
- English.
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In: American Journal of Plant Sciences. - : Scientific Research Publishing, Inc.. - 2158-2742 .- 2158-2750. ; 5, s. 528-534
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Abstract
Subject headings
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- Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing highamounts of several bioactive compounds with nutritional and medicinal traits. It is also planted tocontrol soil erosion. A genetic transformation procedure will facilitate studies of the control ofplant development and interactions with symbionts and pathogens, and will provide a tool forplant breeding. Here, we present a particle bombardment method for transforming seabuckthorn.The early stages of induced adventitious shoots from roots were chosen as a novel target tissue forthe transformation procedure. The root system was bombarded with gold particles coated withplasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression,and shoots were regenerated under kanamycin selection. PCR analysis of the regeneratedtransformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kbfragment from the 35S promoter in three shoots from independent transformation events.
Subject headings
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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