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Co-refolding of a f...
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Agback, TatianaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences
(author)
Co-refolding of a functional complex of Dengue NS3 protease and NS2B co-factor domain and backbone resonance assignment by solution NMR
- Article/chapterEnglish2017
Publisher, publication year, extent ...
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Elsevier BV,2017
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Elsevier,2024
Numbers
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LIBRIS-ID:oai:slubar.slu.se:93161
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https://res.slu.se/id/publ/93161URI
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https://doi.org/10.1016/j.pep.2017.07.002DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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A novel approach for separate expression of dengue virus NS3 protease and its NS2B cofactor domain is described in this paper. The two proteins are expressed in E.coli and purified separately and subsequently efficiently co-refolded to form a stable complex. This straightforward and robust method allows for separate isotope labeling of the two proteins, facilitating analysis by nuclear magnetic resonance (NMR) spectroscopy. Unlinked NS2B-NS3pro behaves better in NMR spectroscopy than linked NS2B-NS3pro, which has resulted in the backbone resonance assignment of the unlinked NS2B-NS3 complex bound to a peptidic boronic acid inhibitor. (C) 2017 Elsevier Inc. All rights reserved.
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Agback, PeterSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences(Swepub:slu)48657
(author)
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Sveriges lantbruksuniversitetInstitutionen för Molekylära vetenskaper
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Related titles
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In:Protein Expression and Purification: Elsevier BV140, s. 16-271046-59281096-0279
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