| 1. |
- Benedet Perea, Susana, 1972, et al.
(author)
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Pituitary and plasma growth hormone dynamics during sexual maturation of female Atlantic salmon
- 2010
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In: GENERAL AND COMPARATIVE ENDOCRINOLOGY. - 0016-6480. ; 167:1, s. 77-85
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Journal article (peer-reviewed)abstract
- Growth hormone in fish regulates many important physiological processes including growth, metabolism and potentially reproduction. In salmonid fish, GH secretion is episodic with irregularly spaced GH peaks. Plasma GH reflects secretion episodes as well as the clearance rate of the hormone, and plasma levels may thus not always reflect the level of activation of the GH axis. This study measured the production dynamics of GH over a 17-month period in sexually maturing female Atlantic salmon which included final maturation and spawning. For the first time, the level of pituitary GH mRNA, pituitary GH protein and plasma GH protein were analyzed concurrently in the same individuals. mRNA and protein were extracted in parallel from the same samples with subsequent real time quantitative PCR to measure mRNA transcripts and radioimmunoassay to measure pituitary and plasma GH protein. Further, the effects of photoperiod manipulation on these parameters were studied. The results show no correlation between mRNA and protein levels except at some time points, and indicate that it is inappropriate to correlate pooled temporal data and averages in time series unless the relationship among the variables is stable over time. The results indicate complex and shifting relationships between pituitary GH mRNA expression, pituitary GH content and plasma GH levels, which could result from changes in clearance rather than secretion rate at different times and its episodic secretion. The study also suggests that there is a functionally important activation of the GH system during spring leading up to maturation and spawning.
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| 2. |
- Le Guyader, Francoise S, et al.
(author)
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Detection of noroviruses in raspberries associated with a gastroenteritis outbreak
- 2004
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In: International Journal of Food Microbiology. - : Elsevier BV. - 0168-1605 .- 1879-3460. ; 97:2, s. 179-186
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Journal article (peer-reviewed)abstract
- Following an acute foodborne gastroenteritis outbreak in southern Sweden, stool specimens from five of nine ill patients were found positive for norovirus using reverse transcriptase polymerase chain reaction. Epidemiological data pointed to raspberry cakes as the source of the outbreak. Using a combination of generic and patient-specific primers and novel food analysis methodology (with extraction efficiency control and inhibitor removal), norovirus strains from two different genogroups were directly identified in the contaminated raspberries.
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| 3. |
- Mittelholzer, Christian, et al.
(author)
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Molecular characterization of a novel astrovirus associated with disease in mink
- 2003
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In: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 84:11, s. 3087-3094
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Journal article (peer-reviewed)abstract
- Pre-weaning diarrhoea is a well-known problem in mink farming in Europe, causing morbidity that varies between farms, regions and season. Different causalities for the disease have been proposed, but only most recently has a novel astrovirus been identified as an important risk factor. In this report, the molecular characterization, origin and evolution of this novel astrovirus of mink are discussed. The polyadenylated, positive-stranded RNA genome was sequenced and found to contain 6610 nt, organized into three ORFs and two short UTRs. A ribosomal frameshift sequence links the 5' two ORFs, containing sequence motifs for a serine protease (ORF1a) and an RNA-dependent RNA polymerase (ORF1b). The structural proteins are encoded by ORF2 and, presumably, are expressed as a polyprotein precursor to be cleaved into the mature capsid proteins. These results indicate that mink astrovirus (MiAstV) has all of the features typical of members of the Astroviridae. Phylogenetic analyses revealed that MiAstV is distantly related to established astroviruses, showing less than 67 % similarity at the nucleotide level with its closest relative, ovine astrovirus, and even lower identities at the predicted amino acid level. Nevertheless, sequence analysis of MiAstV isolates from geographically distinct Swedish and Danish farms showed much less diversity. This suggests either the spread in the mink population of a virus that has evolved a long time ago or the recent introduction of an ancient virus into a new host species.
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| 4. |
- Olofsson, Anders, et al.
(author)
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Unusual, high genetic diversity of Aleutian mink disease virus
- 1999
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In: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 37:12, s. 4145-4149
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Journal article (peer-reviewed)abstract
- The genetic diversity of Aleutian mink disease virus (AMDV) was examined. Sequences obtained from 35 clinical samples were compared with five published sequences. An unusual, high genetic variability was revealed. Three phylogenetic subgroups of AMDV were identified, and the presence of more than one genotype at some farms was detected
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| 5. |
- Rubilar-Abreu, Elba, et al.
(author)
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Serotype G9 rotavirus infections in adults in Sweden
- 2005
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In: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 43:3, s. 1374-1376
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Journal article (peer-reviewed)abstract
- Rotavirus is a major cause of acute gastroenteritis. By examining 1,517 stool samples collected in 2001 and 2002 from Swedish adults with acute diarrhea, rotavirus was found in 3.2%, with the emerging G9P[8] serotype being the one most commonly identified (42.9%). This is the first documentation of G9 infections in adults in Europe. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
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