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Specific modificati...
Specific modification of heparan sulphate is required for normal cerebral cortical development
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McLaughlin, David (författare)
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- Karlsson, Fredrik, 1977- (författare)
- a Division of Biomedical Sciences, The University of Edinburgh, Hugh Robson Building, George Square, Edinburgh
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Tian, Natasha (författare)
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Pratt, Thomas (författare)
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Bullock, Simon L. (författare)
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Wilson, Valerie A. (författare)
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Price, David J. (författare)
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Mason, John O. (författare)
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(creator_code:org_t)
- Elsevier, 2003
- 2003
- Engelska.
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Ingår i: Mechanisms of Development. - : Elsevier. - 0925-4773 .- 1872-6356. ; 120:12, s. 1481-1488
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Proteoglycans are cell surface and extracellular matrix molecules to which long, unbranched glycosaminoglycan side chains are attached. Heparan sulphate, a type of glycosaminoglycan chain, has been proposed as a co-factor necessary for signalling by a range of growth factors. Here we provide evidence that loss of 2-O-sulphation in heparan sulphate leads to a significant reduction in cell proliferation in the developing cerebral cortex. The gene encoding heparan sulphate 2-sulphotransferase (Hs2st) is expressed in embryonic cortex and histological analysis of mice homozygous for a null mutation in Hs2st indicated a reduction in the thickness of the embryonic cerebral cortex. Using 5′-bromodeoxyuridine (BrdU) incorporation assays we found a reduction of approximately 40% in labelling indices of cortical precursor cells at E12. Comparison of the fates of cortical cells born on E13 and E15 in Hs2st−/− mutant and wildtype littermate embryos revealed no differences in the pattern of cell migration. Our findings suggest a critical role for 2-O-sulphation of heparan sulphate proteoglycan (HSPG) in regulating cell proliferation during development of the cerebral cortex, perhaps through the modulation of cellular responses to growth factor signalling.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Growth factors
- Heparan sulphate proteoglycans
- Heparan sulphate 2-sulphotransferase
- Neural development
- Proliferation
- Telencephalon
- BrdU
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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