Tyck till om SwePub Sök
här!
Sökning: onr:"swepub:oai:DiVA.org:gih-1104" >
Ultra-endurance exe...
Ultra-endurance exercise increases the production of reactive oxygen species in isolated mitochondria from human skeletal muscle.
-
- Sahlin, Kent (författare)
- Karolinska Institutet,Gymnastik- och idrottshögskolan,Forskningsgruppen Mitokondriell funktion och metabol kontroll
-
- Shabalina, Irina G (författare)
- Stockholms universitet,Gymnastik- och idrottshögskolan,Åstrandlaboratoriet,Stockholm University,Avdelningen för fysiologi
-
- Mattsson, C Mikael (författare)
- Karolinska Institutet,Gymnastik- och idrottshögskolan,Björn Ekbloms forskningsgrupp
-
visa fler...
-
- Bakkman, Linda (författare)
- Gymnastik- och idrottshögskolan,Åstrandlaboratoriet,Karolinska Institutet
-
- Fernström, Maria (författare)
- Gymnastik- och idrottshögskolan,Åstrandlaboratoriet,Karolinska Institutet
-
- Rozhdestvenskaya, Zinaida (författare)
- Gymnastik- och idrottshögskolan,Åstrandlaboratoriet
-
- Enqvist, Jonas K (författare)
- Gymnastik- och idrottshögskolan,Björn Ekbloms forskningsgrupp
-
- Nedergaard, Jan (författare)
- Stockholms universitet,Avdelningen för fysiologi,Stockholm University
-
- Ekblom, Björn T (författare)
- Gymnastik- och idrottshögskolan,Björn Ekbloms forskningsgrupp
-
- Tonkonogi, Michail (författare)
- Högskolan Dalarna,Gymnastik- och idrottshögskolan,Åstrandlaboratoriet,University of Dalarna,Medicinsk vetenskap
-
visa färre...
-
(creator_code:org_t)
- American Physiological Society, 2010
- 2010
- Engelska.
-
Ingår i: Journal of applied physiology. - : American Physiological Society. - 8750-7587 .- 1522-1601. ; 108:4, s. 780-787
- Relaterad länk:
-
https://europepmc.or...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://urn.kb.se/re...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Exercise-induced oxidative stress is important for the muscular adaptation to training but may also cause muscle damage. We hypothesized that prolonged exercise would increase mitochondrial production of reactive oxygen species (ROS) measured in vitro and that this correlates with oxidative damage. Eight male athletes (24-32 years) performed ultra-endurance exercise (kayaking/running/cycling) with an average work intensity of 55% VO2peak for 24 h. Muscle biopsies were taken from vastus lateralis before exercise, immediately after exercise and after 28 h of recovery. The production of H2O2 was measured fluorometrically in isolated mitochondria with the Amplex red and peroxidase system. Succinate-supported mitochondrial H2O2 production was significantly increased after exercise (73% higher, P=0.025) but restored to the initial level at recovery. Plasma level of free fatty acids (FFA) increased 4-fold and exceeded 1.2 mmol l(-1) during the last 6 h of exercise. Plasma FFA at the end of exercise was significantly correlated to mitochondrial ROS production (r=0.74, P<0.05). Mitochondrial content of 4-hydroxy-nonenal-adducts (a marker of oxidative damage) was increased only after recovery and was not correlated with mitochondrial ROS production. Total thiol-group level and glutathione peroxidase activity were elevated after recovery. In conclusion: ultra-endurance exercise increases ROS production in isolated mitochondria but this is reversed after 28 h recovery. Mitochondrial ROS production was not correlated with oxidative damage of mitochondrial proteins, which was increased at recovery but not immediately after exercise. Key words: antioxidative defence, fatty acids, oxidative stress.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Idrottsvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Sport and Fitness Sciences (hsv//eng)
Nyckelord
- antioxidative defence
- fatty acids
- oxidative stress
- Physiology
- Fysiologi
- Medicin/Teknik
- Medicine/Technology
- NATURAL SCIENCES
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas