Evolution reveals a glutathione-dependent mechanism of 3-hydroxypropionic acid tolerance

Kildegaard, Kanchana R.Danmarks Tekniske Universitet,Technical University of Denmark (författare)

QC 20150126
Biologically produced 3-hydroxypropionic acid (3HP) is a potential source for sustainable acrylates and can also find direct use as monomer in the production of biodegradable polymers. For industrial scale production there is a need for robust cell factories tolerant to high concentration of 3HP, preferably at low pH. Through adaptive laboratory evolution we selected S. cerevisiae strains with improved tolerance to 3HP at pH 3.5. Genome sequencing followed by functional analysis identified the causal mutation in SFA1 gene encoding S-(hyclroxymerhyl)glutathione dehydrogenase. Based on our findings, we propose that 3HP toxicity is mediated by 3-hydroxypropionic aldehyde (reuterin ) and that glutathione-dependent reactions are used for reuterin detoxification. The identified molecular response to 3HP and reuterin may well be a general mechanism for handling resistance to organic acid and aldehydes by living cells. (C) 2014 International Metabolic Engineering Society Published by Elsevier Inc. On behalf of International Metabolic Engineering Society. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/)

Hallström, Björn M.KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)(Swepub:kth)u1ih19oh (författare)
Blicher, Thomas H.Köpenhamns universitet,University of Copenhagen (författare)
Sonnenschein, NikolausDanmarks Tekniske Universitet,Technical University of Denmark (författare)
Jensen, Niels B.Danmarks Tekniske Universitet,Technical University of Denmark (författare)
Sherstyk, SvetlanaDanmarks Tekniske Universitet,Technical University of Denmark (författare)
Harrison, Scott J. (författare)
Maury, JeromeDanmarks Tekniske Universitet,Technical University of Denmark (författare)
Herrgard, Markus J. (författare)
Juncker, Agnieszka S. (författare)
Forster, JochenDanmarks Tekniske Universitet,Technical University of Denmark (författare)
Nielsen, Jens B,1962Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)nielsenj (författare)
Borodina, IrinaDanmarks Tekniske Universitet,Technical University of Denmark (författare)
Danmarks Tekniske UniversitetProteomik och nanobioteknologi (creator_code:org_t)

Av författaren/redakt...: Kildegaard, Kanc ...; Hallström, Björn ...; Blicher, Thomas ...; Sonnenschein, Ni ...; Jensen, Niels B.; Sherstyk, Svetla ...; visa fler...; Harrison, Scott ...; Maury, Jerome; Herrgard, Markus ...; Juncker, Agniesz ...; Forster, Jochen; Nielsen, Jens B, ...; Borodina, Irina; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.