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Single-cell charact...
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Tauriainen, JohannaKarolinska Institutet
(författare)
Single-cell characterization of in vitro migration and interaction dynamics of T cells expanded with IL-2 and IL-7
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
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2015-04-28
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Frontiers Media SA,2015
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-169286
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-169286URI
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https://doi.org/10.3389/fimmu.2015.00196DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:131292709URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20150617
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T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response against cancer cells, T cells need to migrate to the cancer-site, engage in contacts with cancer cells, and perform their effector functions. Adoptive T cell therapy is an effective strategy as treatment of complications such as relapse or opportunistic infections after hematopoietic stem cell transplantations. This requires a sufficient amount of cells that are able to expand and respond to tumor or viral antigens. The cytokines interleukin (IL)-2 and IL-7 drive T cell differentiation, proliferation, and survival and are commonly used to expand T cells ex vivo. Here, we have used microchip-based live-cell imaging to follow the migration of individual T cells, their interactions with allogeneic monocytes, cell division, and apoptosis for extended periods of time; something that cannot be achieved by commonly used methods. Our data indicate that cells grown in IL-7 + IL-2 had similar migration and contact dynamics as cells grown in IL-2 alone. However, the addition of IL-7 decreased cell death creating a more viable cell population, which should be beneficial when preparing cells for immunotherapy.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Gustafsson, KarinKTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1ucx9jd
(författare)
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Göthlin, MårtenKTH,Tillämpad fysik,Science for Life Laboratory, SciLifeLab(Swepub:kth)-
(författare)
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Gertow, Jens
(författare)
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Buggert, MarcusKarolinska Institutet
(författare)
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Frisk, Thomas W.KTH,Cellens fysik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1l00se6
(författare)
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Karlsson, Annika C.Karolinska Institutet
(författare)
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Uhlin, MichaelKarolinska Institutet
(författare)
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Önfelt, BjörnKTH,Cellens fysik,Science for Life Laboratory, SciLifeLab,Karolinska Institutet, Sweden(Swepub:kth)u1tquq43
(författare)
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Karolinska InstitutetTillämpad fysik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Frontiers in Immunology: Frontiers Media SA61664-3224
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