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Genotyping by dynam...
Genotyping by dynamic heating of monolayered beads on a microheater surface
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- Russom, Aman (författare)
- KTH,Mikrosystemteknik
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- Haasl, Sjoerd (författare)
- KTH,Mikrosystemteknik
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- Ohlander, Anna (författare)
- KTH,Mikrosystemteknik
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visa fler...
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- Mayr, T. (författare)
- Karolinska Institute
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- Brookes, A. J. (författare)
- Karolinska Institute
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- Andersson, Helene (författare)
- KTH,Mikrosystemteknik
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- Stemme, Göran (författare)
- KTH,Mikrosystemteknik
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visa färre...
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(creator_code:org_t)
- Royal Society of Chemistry, 2005
- 2005
- Engelska.
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Ingår i: Micro Total Analysis Systems 2004. - : Royal Society of Chemistry. - 0854048960 ; , s. 303-305
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- This paper presents SNP scoring by DASH technology by employing dynamic heating of beads immobilized on a chip with integrated heater and sensor. The microfabricated chip designed for open-surface DNA analysis allows fast, well controllable temperature ramping and homogeneous temperature distribution over the entire heater area. Beads containing DNA duplexes are immobilized on the surface of the chip by microcontact printing using a PDMS stamp. All three possible variants of a SNP site of an oligonucleotide were accurately scored using the bead-based DASH approach. Using the chip, the total analysis time could easily be reduced by a factor 2 compared with the current DASH assay.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Nyckelord
- DASH
- microheater
- microcontact printing
- microspheres
- SNP
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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