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Model-based design ...
Model-based design and control of a small-scale integrated continuous end-to-end mAb platform
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- Gomis-Fons, Joaquín (författare)
- Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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Schwarz, Oliver, 1984- (författare)
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- Zhang, Liang (författare)
- KTH Royal Institute of Technology,KTH,Industriell bioteknologi
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- Andersson, Niklas (författare)
- Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Nilsson, Bernt (författare)
- Lund University,Lunds universitet,Avdelningen för kemiteknik,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Chemical Engineering,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Castan, A. (författare)
- GE Healthcare Bio-Sciences AB (GEHC)
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- Solbrand, A. (författare)
- GE Healthcare Bio-Sciences AB (GEHC)
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- Stevenson, J. (författare)
- Cobra Biologics
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- Chotteau, Véronique, Docent, 1963- (författare)
- KTH Royal Institute of Technology,KTH,Industriell bioteknologi
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- Schwarz, Hubert (författare)
- KTH Royal Institute of Technology
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(creator_code:org_t)
- 2020-04-06
- 2020
- Engelska.
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Ingår i: Biotechnology progress (Print). - : John Wiley and Sons Inc.. - 8756-7938 .- 1520-6033.
- Relaterad länk:
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https://doi.org/10.1...
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https://onlinelibrar...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- A continuous integrated bioprocess available from the earliest stages of process development allows for an easier, more efficient and faster development and characterization of an integrated process as well as production of small-scale drug candidates. The process presented in this article is a proof-of-concept of a continuous end-to-end monoclonal antibody production platform at a very small scale based on a 200 ml alternating tangential flow filtration perfusion bioreactor, integrated with the purification process with a model-based design and control. The downstream process, consisting of a periodic twin-column protein A capture, a virus inactivation, a CEX column and an AEX column, was compactly implemented in a single chromatography system, with a purification time of less than 4 hr. Monoclonal antibodies were produced for 17 days in a high cell density perfusion culture of CHO cells with titers up to 1.0 mg/ml. A digital twin of the downstream process was created by modelling all the chromatography steps. These models were used for real-time decision making by the implementation of control strategies to automatize and optimize the operation of the process. A consistent glycosylation pattern of the purified product was ensured by the steady state operation of the process. Regarding the removal of impurities, at least a 4-log reduction in the HCP levels was achieved. The recovery yield was up to 60%, and a maximum productivity of 0.8 mg/ml/day of purified product was obtained.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Kemiska processer (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Chemical Process Engineering (hsv//eng)
Nyckelord
- end-to-end continuous bioprocessing
- monoclonal antibody
- perfusion bioreactor
- process integration
- process modelling
- Bioreactors
- Column chromatography
- Controlled drug delivery
- Decision making
- Digital twin
- Monoclonal antibodies
- Purification
- Removal
- Viruses
- Continuous bioprocessing
- Monoclonal antibody production
- Real time decision-making
- Steady-state operation
- Tangential flow filtrations
- Process control
- end-to-end continuous bioprocessing
- monoclonal antibody
- perfusion bioreactor
- process integration
- process modelling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Gomis-Fons, Joaq ...
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Schwarz, Oliver, ...
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Zhang, Liang
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Andersson, Nikla ...
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Nilsson, Bernt
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Castan, A.
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visa fler...
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Solbrand, A.
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Stevenson, J.
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Chotteau, Véroni ...
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Schwarz, Hubert
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visa färre...
- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Bioprocessteknik
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Kemiteknik
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och Kemiska processe ...
- Artiklar i publikationen
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Biotechnology pr ...
- Av lärosätet
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Kungliga Tekniska Högskolan
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Lunds universitet