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Multiplexed Microfl...
Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production
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- Pinto, Ines Fernandes (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Soares, Ruben R. G. (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Mäkinen, Meeri (författare)
- KTH,Industriell bioteknologi,Centre for Advanced BioProduction by Continuous Processing, AdBIOPRO
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- Chotteau, Veronique (författare)
- KTH,Industriell bioteknologi,Centre for Advanced BioProduction by Continuous Processing, AdBIOPRO
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- Russom, Aman, Prof. 1976- (författare)
- Karolinska Institutet,KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab,AIMES, Center for the Advancement of Integrated Medical and Engineering Sciences, Karolinska Institutet
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(creator_code:org_t)
- 2021-03-16
- 2021
- Engelska.
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Ingår i: ACS Sensors. - : American Chemical Society (ACS). - 2379-3694. ; 6:3, s. 842-851
- Relaterad länk:
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https://doi.org/10.1...
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https://kth.diva-por... (primary) (Raw object)
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https://pubs.acs.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The biopharmaceutical market has been rapidly growing in recent years, creating a highly competitive arena where R&D is critical to strike a balance between clinical safety and profitability. Toward process optimization, the recent development and adoption of new process analytical technologies (PAT) highlight the dynamic complexity of mammalian/human cell culture processes, as well as the importance of fine-tuning and modeling key metabolites and proteins. In this context, simple, rapid, and cost-effective devices allowing routine at-line monitoring of specific proteins during process development and production are currently lacking. Here, we report the development of a versatile microfluidic protein analysis cartridge allowing the multiplexed bead-based immunodetection of specific proteins directly from complex mixtures with minimal hands-on time. Colorimetric quantification of Chinese hamster ovary (CHO) host cell proteins as key impurities, monoclonal antibodies as target biopharmaceuticals, and lactate dehydrogenase as a marker of cell viability was achieved with limits of detection in the 1-10 ng/mL range and analysis times as short as 30 min. The device was further demonstrated for the monitoring of a Rituximab-producing CHO cell bioreactor over the course of 8 days, providing comparable recoveries to standard enzyme-linked immunosorbent assay (ELISA) kits. The high sensitivity combined with robustness to matrix interference highlights the potential of the device to perform at-line measurements spanning from the bioreactor to the downstream processing.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Nyckelord
- microfluidics
- streptavidin beads
- immunoassay
- colorimetric
- monoclonal antibodies
- host cell proteins
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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