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- Akhtar, Ahmad SaleemKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab (författare)
A portable and low-cost centrifugal microfluidic platform for multiplexed colorimetric detection of protein biomarkers
- Artikel/kapitelEngelska2023
Förlag, utgivningsår, omfång ...
- Elsevier BV,2023
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:kth-323338
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-323338URI
- https://doi.org/10.1016/j.aca.2023.340823DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:151784113URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- QC 20230307
- Cytokines play a very important role in our immune system by acting as mediators to put up a coordinated defense against foreign elements in our body. Elevated levels of cytokines in the body can signal to an ongoing response of the immune system to some abnormality. Thus, the quantification of a panel of cytokines can provide valuable information regarding the diagnosis of specific diseases and state of overall health of an individual. Conventional Enzyme Linked Immunosorbent Assay (ELISA) is the gold-standard for quantification of cytokines, however the need for trained personnel and expensive equipment limits its application to centralized laboratories only. In this context, there is a lack of simple, low-cost and portable devices which can allow for quantification of panels of cytokines at point-of-care and/or resource limited settings.Here, we report the development of a versatile, low-cost and portable bead-based centrifugal microfluidic platform allowing for multiplexed detection of cytokines with minimal hands-on time and an integrated colorimetric signal readout without the need for any external equipment. As a model, multiplexed colorimetric quantification of three target cytokines i.e., Tumor necrosis factor alpha (TNF-α), Interferon gamma (IFN-γ) and Interleukin-2 (IL-2) was achieved in less than 30 min with limits of detection in ng/mL range. The developed platform was further evaluated using spiked-in plasma samples to test for matrix interference. The ease of use, low-cost and portability of the developed platform highlight its potential to serve as a sample-to-answer solution for detection of cytokine panels in resource limited settings.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
- Lab-on-a-disc
- Photodetectors
- Immunoassay
- Cytokines
- Point-of-care
- Resource limited settings
- Teknik och hälsa
- Technology and Health
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Soares, Ruben R. G.KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi(Swepub:kth)u166jk6w (författare)
- Pinto, Ines FernandesKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1g105sd (författare)
- Russom, Aman,Prof.1976-KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES(Swepub:kth)u1go6jxm (författare)
- KTHNanobioteknologi (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Analytica Chimica Acta: Elsevier BV12450003-26701873-4324
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