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Itch receptor MRGPR...
Itch receptor MRGPRX4 interacts with the receptor activity-modifying proteins
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- Kotliar, Ilana B. (författare)
- Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA.;Triinst PhD Program Chem Biol, New York, NY USA.
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- Ceraudo, Emilie (författare)
- Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA.
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- Kemelmakher-Liben, Kevin (författare)
- Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA.
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- Oren, Deena A. (författare)
- Rockefeller Univ, Struct Biol Resource Ctr, New York, NY USA.
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- Lorenzen, Emily (författare)
- Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA.
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- Dodig-Crnkovic, Tea (författare)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab,Publ Hlth Agcy Sweden, Dept Microbiol, Solna, Sweden.
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- Horioka-Duplix, Mizuho (författare)
- Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA.;Exscientia, Schrodinger Bldg,Oxford Sci Park, Oxford, England.
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- Huber, Thomas (författare)
- Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA.
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- Schwenk, Jochen M. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Sakmar, Thomas P. (författare)
- Karolinska Institutet
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Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA;Triinst PhD Program Chem Biol, New York, NY USA. Rockefeller Univ, Lab Chem Biol & Signal Transduct, New York, NY 10065 USA. (creator_code:org_t)
- Elsevier BV, 2023
- 2023
- Engelska.
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Ingår i: Journal of Biological Chemistry. - : Elsevier BV. - 0021-9258 .- 1083-351X. ; 299:5
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Cholestatic itch is a severe and debilitating symptom in liver diseases with limited treatment options. The class A G proteincoupled receptor (GPCR) Mas-related GPCR subtype X4 (MRGPRX4) has been identified as a receptor for bile acids, which are potential cholestatic pruritogens. An increasing number of GPCRs have been shown to interact with receptor activity-modifying proteins (RAMPs), which can modulate different aspects of GPCR biology. Using a combination of multiplexed immunoassay and proximity ligation assay, we show that MRGPRX4 interacts with RAMPs. The interaction of MRGPRX4 with RAMP2, but not RAMP1 or 3, causes attenuation of basal and agonist-dependent signaling, which correlates with a decrease of MRGPRX4 cell surface expression as measured using a quantitative NanoBRET pulse-chase assay. Finally, we use AlphaFold Multimer to predict the structure of the MRGPRX4-RAMP2 complex. The discovery that RAMP2 regulates MRGPRX4 may have direct implications for future drug development for cholestatic itch.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
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