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Effect of substrate...
Effect of substrate feed rate on recombinant protein secretion, degradation and invlusion body formation in Escherichia coli
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- Boström, Maria (författare)
- KTH,Skolan för bioteknologi (BIO)
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- Markland, Katrin (författare)
- KTH,Centrum för Bioprocessteknik, CBioPT
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- Sandén, Anna Maria (författare)
- KTH,Centrum för Bioprocessteknik, CBioPT
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- Hedhammar, My (författare)
- KTH,Proteomik,Centrum för Bioprocessteknik, CBioPT
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- Hober, Sophia (författare)
- KTH,Proteomik,Centrum för Bioprocessteknik, CBioPT
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- Larsson, Gen (författare)
- KTH,Centrum för Bioprocessteknik, CBioPT
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(creator_code:org_t)
- 2005-01-18
- 2005
- Engelska.
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Ingår i: Applied Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 0175-7598 .- 1432-0614. ; 68:1, s. 82-90
- Relaterad länk:
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http://www.springerl...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The effect of changes in substrate feed rate during fedbatch cultivation was investigated with respect to soluble protein formation and transport of product to the periplasm in Escherichia coli. Production was transcribed from the P-malK promoter; and the cytoplasmic part of the production was compared with production from the P-lacUV5 promoter. The fusion protein product, Zb-MalE, was at all times accumulated in the soluble protein fraction except during high-feed-rate production in the cytoplasm. This was due to a substantial degree of proteolysis in all production systems, as shown by the degradation pattern of the product. The product was also further subjected to inclusion body fori-nation. Production in the periplasm resulted in accumulation of the full-length protein; and this production system led to a cellular physiology where the stringent response could be avoided. Furthermore, the secretion could be used to abort the diauxic growth phase resulting from use of the P-malK promoter. At high feed rate, the accumulation of acetic acid, due to overflow metabolism, could furthermore be completely avoided.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- fed-batch production
- cultivations
- expression
- promoters
- fusion
- system
- Biochemistry
- Biokemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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