Sökning: onr:"swepub:oai:DiVA.org:kth-91800" >
Ultra-rapid melting...
Ultra-rapid melting curve analysis on beads for high-throughput genotyping of single nucleotide polymorphism
-
- Russom, Aman (författare)
- KTH,Mikrosystemteknik
-
- Haasl, Sjoerd (författare)
- KTH,Mikrosystemteknik
-
Brookes, Anthony J. (författare)
-
visa fler...
-
- Andersson, Helene (författare)
- KTH,Mikrosystemteknik
-
- Stemme, Göran (författare)
- KTH,Mikrosystemteknik
-
visa färre...
-
(creator_code:org_t)
- Transducers Research Foundations, 2005
- 2005
- Engelska.
-
Ingår i: Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference. - : Transducers Research Foundations. - 9780974361116 ; , s. 1006-1008
- Relaterad länk:
-
https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- This report describes a rapid solid-phase melting curve analysis method based on dynamic allele-specific hybridization (DASH) for single nucleotide polymorphism (SNP) genotyping. Beads with DNA duplexes are immobilized on the surface of a microheater chip. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Hence, analyses that took more than 15 minutes could be performed in less than 1 minute, enabling ultra-rapid SNP analysis.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Annan teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies (hsv//eng)
Nyckelord
- Beads
- DASH
- Microheater
- Single nucleotide polymorphism
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
Hitta via bibliotek
Till lärosätets databas