Comparison and Functionalization Study of Microemulsion-Prepared Magnetic Iron Oxide Nanoparticles

• Magnetic iron oxide nanoparticles (MION) for protein binding and separation were obtained from water-in-oil (w/o) and oil-in-water (o/w) microemulsions. Characterization of the prepared nanoparticles have been performed by TEM, XRD, SQUID magnetometry, and BET. Microemulsion-prepared magnetic iron oxide nanoparticles (ME-MION) with sizes ranging from 2 to 10 rim were obtained. Study on the magnetic properties at 300 K shows a large increase of the magnetization similar to 35 emu/g for w/o-ME-MION with superparamagnetic behavior and nanoscale dimensions in comparison with o/w-ME-MION (10 emu/g) due to larger particle size and anisotropic property. Moringa oleifera coagulation protein (MOCP) bound w/o- and o/w-ME-MION showed an enhanced performance in terms of coagulation activity. A significant interaction between the magnetic nanoparticles and the protein can be described by changes in fluorescence emission spectra. Adsorbed protein from MOCP is still retaining its functionality even after binding to the nanoparticles, thus implying the extension of this technique for various applications.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Nanoteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Nano-technology (hsv//eng)

Nyckelord

Anisotropic property

Coagulation proteins

Fluorescence emission spectra

Functionalizations

Larger particle sizes

Magnetic iron oxide nanoparticles

Magnetic nanoparticles

Moringa oleifera

Nanoscale dimensions

Oil-in-water

Protein binding

SQUID magnetometry

Superparamagnetic behavior

Water-in-oil

XRD

Biochemistry

Coagulation

Emission spectroscopy

Magnetic properties

Metal nanoparticles

Proteins

Superparamagnetism

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