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Two-dimensional mic...
Two-dimensional micro array fluorescence fingerprinting with a computer screen photo-assisted technique
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- Bakker, Jimmy W.P. (författare)
- Linköpings universitet,Tillämpad optik,Tekniska högskolan
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- Filippini, Daniel (författare)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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- Lundström, Ingemar (författare)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
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(creator_code:org_t)
- SPIE, 2005
- 2005
- Engelska.
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Ingår i: Spectral Imaging. - : SPIE. ; , s. 9-15
- Relaterad länk:
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http://urn.kb.se/res...
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visa fler...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Detection and classification of fluorescent dyes are demonstrated using a computer screen photo-assisted technique (CSPT). This technique has previously been demonstrated for analyzing fluorescence from 96 wells microtiterplates (200 µl per well) and from a single cuvette with some optics to enhance sensitivity. In this work a custom designed array of wells with a volume of approximately 1 mu;l is used. In order to measure such small volumes without saturating the detector, the transmitted light is masked by placing the sample between two crossed polarizers. This arrangement blocks nearly all the transmitted light, while the emitted light, which is nearly unpolarized, can still be detected. The lowest amount (concentration x volume) of analyte detectable in this setup is about 40 times smaller than in the previous setups.
Nyckelord
- NATURAL SCIENCES
- NATURVETENSKAP
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