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Establishing and va...
Establishing and validating the fluorescent amyloid ligand h-FTAA (heptamer formyl thiophene acetic acid) to identify transthyretin amyloid deposits in carpal tunnel syndrome
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- Hahn, Katharina (författare)
- Christian Albrechts University of Kiel, Germany
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- Nilsson, Peter (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Hammarström, Per (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Urban, Peter (författare)
- Institute Pathol and Dermatopathol, Germany
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- Ruediger Meliss, Rolf (författare)
- Institute Dermatopathol, Germany
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- Behrens, Hans-Michael (författare)
- Christian Albrechts University of Kiel, Germany
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- Krueger, Sandra (författare)
- Christian Albrechts University of Kiel, Germany
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- Roecken, Christoph (författare)
- Christian Albrechts University of Kiel, Germany
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(creator_code:org_t)
- 2017-04-23
- 2017
- Engelska.
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Ingår i: Amyloid. - : TAYLOR & FRANCIS LTD. - 1350-6129 .- 1744-2818. ; 24:2, s. 78-86
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Transthyretin-derived (ATTR) amyloidosis is a frequent finding in carpal tunnel syndrome. We tested the following hypotheses: the novel fluorescent amyloid ligand heptameric formic thiophene acetic acid (h-FTAA) has a superior sensitivity for the detection of amyloid compared with Congo red-staining; Amyloid load correlates with patient gender and/or patient age. We retrieved 208 resection specimens obtained from 184 patients with ATTR amyloid in the carpal tunnel. Serial sections were stained with Congo red, h-FTAA and an antibody directed against transthyretin (TTR). Stained sections were digitalized and forwarded to computational analyses. The amount of amyloid was correlated with patient demographics. Amyloid stained intensely with h-FTAA and an anti-TTR-antibody. Congo red-staining combined with fluorescence microscopy was significantly less sensitive than h-FTAA-fluorescence and TTR-immunostaining: the highest percentage area was found in TTR-immunostained sections, followed by h-FTAA and Congo red. The Pearson correlation coefficient was .8 (Congo red vs. h-FTAA) and .9 (TTR vs. h-FTAA). Amyloid load correlated with patient gender, anatomical site and patient age. h-FTAA is a highly sensitive method to detect even small amounts of ATTR amyloid in the carpal tunnel. The staining protocol is easy and h-FTAA may be a much more sensitive procedure to detect amyloid at an earlier stage.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Nyckelord
- Amyloid; Congo red; ATTR; transthyretin; h-FTAA; carpal tunnel
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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