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Processable enzyme-...
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Liu, YuLinköpings universitet,Kemi,Tekniska fakulteten,Sichuan Agriculture University, Peoples R China
(författare)
Processable enzyme-hybrid conductive polymer composites for electrochemical biosensing
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
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ELSEVIER ADVANCED TECHNOLOGY,2018
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LIBRIS-ID:oai:DiVA.org:liu-143600
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-143600URI
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https://doi.org/10.1016/j.bios.2017.09.021DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Funding Agencies|Swedish Research Council [VR-2015-04434]; China Scholarship Council (CSC) [201406910068]
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A new approach for the facile fabrication of electrochemical biosensors using a biohybrid conducting polymer was demonstrated using glucose oxidase (GOx) and poly (3, 4-ethylenedioxythiophene) (PEDOT) as a model. The biohybrid conducting polymer was prepared based on a template-assisted chemical polymerisation leading to the formation of PEDOT microspheres (PEDOT-MSs), followed by in-situ deposition of platinum nanoparticles (PtNPs) and electrostatic immobilisation of glucose oxidase (GOx) to form water processable GOx-PtNPs-PEDOT-MSs. The morphology, chemical composition and electrochemical performance of the GOx-PtNPs-PEDOT-MS-based glucose biosensor were characterised using scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDS), Fourier transform infrared (FTIR) spectroscopy, zeta potential and electrochemical measurements, respectively. The biosensor delivered a linear response for glucose over the range 0.1-10 mM (R-2 = 0.9855) with a sensitivity of 116.25 mu A mM(-1) cm(-2), and limit of detection of 1.55 mu M (3 x SD/sensitivity). The sensitivity of the developed PEDOT-MS based biosensor is significantly higher (2.7 times) than the best reported PEDOT-based glucose biosensor in the literature. The apparent Michaelis Menten constant (K-m(app)) of the GOx-PtNPs-PEDOT-MS-based biosensors was calculated as 7.3 mM. Moreover, the biosensor exhibited good storage stability, retaining 97% of its sensitivity after 12 days storage. This new bio-hybrid conducting polymer combines the advantages of micro-structured morphology, compatibility with large-scale manufacturing processes, and intrinsic biocatalytic activity and conductivity, thus demonstrating its potential as a convenient material for printed bioelectronics and sensors.
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Turner, AnthonyLinköpings universitet,Sensor- och aktuatorsystem,Tekniska fakulteten(Swepub:liu)anttu13
(författare)
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Zhao, MaojunSichuan Agriculture University, Peoples R China
(författare)
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Mak, Wing CheungLinköpings universitet,Sensor- och aktuatorsystem,Tekniska fakulteten(Swepub:liu)winma81
(författare)
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Linköpings universitetKemi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biosensors & bioelectronics: ELSEVIER ADVANCED TECHNOLOGY100, s. 374-3810956-56631873-4235
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