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Sökning: onr:"swepub:oai:DiVA.org:liu-145440" > Simple storage (CO2...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04194naa a2200433 4500 | |
| 001 | oai:DiVA.org:liu-145440 | |
| 003 | SwePub | |
| 008 | 180403s2018 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1454402 URI |
| 024 | 7 | a https://doi.org/10.1016/j.theriogenology.2017.12.0012 DOI |
| 040 | a (SwePub)liu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Martinez, C. A.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 245 | 1 0 | a Simple storage (CO2-free) of porcine morulae for up to three days maintains the in vitro viability and developmental competence |
| 264 | 1 | b ELSEVIER SCIENCE INC,c 2018 |
| 338 | a electronic2 rdacarrier | |
| 500 | a Funding Agencies|Ministry of Economy and Competitiveness (Madrid, Spain)/the European Regional Development Fund [RTC-2016-5448-2, AGL2015-69735-R]; Seneca Foundation, Murcia, Spain [19892/GERM/15]; Swedish Research Council Formas, Stockholm, Sweden [2017-00946] | |
| 520 | a The advancement of porcine embryo transfer (ET) technology is constrained by regulatory hurdles (liquid nitrogen transportation) or, more importantly, the technical obstacles of using vitrified embryos in combination with nonsurgical deep uterine ET technology. Maintaining embryos in culture during transport and prior ET collides with the need of CO2 gassing and the best choice of culture medium. In this work, we describe storage conditions for short-term embryo CO2-free storage that allowed for a majority of in vivo-derived porcine morulae to survive after 3 days of storage in a liquid state, and to develop to the blastocyst stage unhatched, a sanitary prerequisite for ET. The storage conditions included NCSU-23 medium supplemented with bovine serum albumin, where bicarbonate was partially replaced by HEPES to avoid the need for CO2 gassing, and a temperature of 37 degrees C. These conditions were able to maintain the functionality of the stored embryos (hatching capacity after exposure to conventional culture conditions) and their developmental competence after ET (normal fetuses by day 38 of pregnancy). Use of this strategy of CO2-free storage should allow the shipment of fresh embryos worldwide in the absence of liquid nitrogen. (C) 2017 Elsevier Inc. All rights reserved. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Reproduktionsmedicin och gynekologi0 (SwePub)302202 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Obstetrics, Gynaecology and Reproductive Medicine0 (SwePub)302202 hsv//eng |
| 653 | a CO2-free embryo storage; Morulae; Blastocyst; Embryo transfer; Pig | |
| 700 | 1 | a Nohalez, A.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 700 | 1 | a Parrilla, I.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 700 | 1 | a Lucas, X.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 700 | 1 | a Sanchez-Osorio, J.u Topigs Norsvin Espana, Spain4 aut |
| 700 | 1 | a Roca, J.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 700 | 1 | a Cuello, C.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 700 | 1 | a Rodriguez-Martinez, Heribertou Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten4 aut0 (Swepub:liu)herro55 |
| 700 | 1 | a Martinez, E. A.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 700 | 1 | a Gil, M. A.u Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain4 aut |
| 710 | 2 | a Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spainb Topigs Norsvin Espana, Spain4 org |
| 773 | 0 | t Theriogenologyd : ELSEVIER SCIENCE INCg 108, s. 229-238q 108<229-238x 0093-691Xx 1879-3231 |
| 856 | 4 | u https://liu.diva-portal.org/smash/get/diva2:1194600/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint |
| 856 | 4 | u http://liu.diva-portal.org/smash/get/diva2:1194600/FULLTEXT01 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-145440 |
| 856 | 4 8 | u https://doi.org/10.1016/j.theriogenology.2017.12.001 |
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