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Antimicrobial susce...
Antimicrobial susceptibility testing of Mycobacterium tuberculosis complex isolates - the EUCAST broth microdilution reference method for MIC determination
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- Schön, Thomas (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Kalmar County Hospital, Sweden
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- Werngren, Jim (författare)
- Publ Hlth Agcy Sweden, Sweden
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- Machado, Diana (författare)
- Univ Nova Lisboa, Portugal
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- Borroni, Emanuele (författare)
- IRCCS San Raffaele Sci Inst, Italy
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- Wijkander, Maria (författare)
- Publ Hlth Agcy Sweden, Sweden
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- Lina, Gerard (författare)
- Univ Lyon 1, France; Hosp Civils Lyon, France
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- Mouton, Johan (författare)
- Erasmus MC, Netherlands
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- Matuschek, Erika (författare)
- Cent Hosp Vaxjo, Sweden; EUCAST Dev Lab, Sweden
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- Kahlmeter, Gunnar (författare)
- Cent Hosp Vaxjo, Sweden; EUCAST Dev Lab, Sweden
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- Giske, Christian (författare)
- Karolinska Institutet
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- Santin, Miguel (författare)
- Univ Barcelona, Spain
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- Cirillo, Daniela Maria (författare)
- IRCCS San Raffaele Sci Inst, Italy
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- Viveiros, Miguel (författare)
- Univ Nova Lisboa, Portugal
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- Cambau, Emmanuelle (författare)
- GHU Nord, France; Univ Paris, France
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(creator_code:org_t)
- ELSEVIER SCI LTD, 2020
- 2020
- Engelska.
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Ingår i: Clinical Microbiology and Infection. - : ELSEVIER SCI LTD. - 1198-743X .- 1469-0691. ; 26:11, s. 1488-1492
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Scope: Several methods are used worldwide for antibiotic susceptibility testing (AST) for the Mycobacterium tuberculosis complex (MTBC). The variability in the results obtained with these methods hampers setting epidemiological cut-off (ECOFF) values and clinical breakpoints according to EUCAST guidelines. Methods for susceptibility testing and determination of the minimal inhibitory concentrations (MICs) need to be standardized for MTBC isolates for old and new agents. Our objective was to establish a standardized reference method for MIC determination for MTBC. Methods: The EUCAST antimycobacterial susceptibility testing subcommittee (AMST) compared protocols of MIC determination with regard to medium, inoculum preparation, antituberculous agent preparation, incubation, reading of the results and interpretation. Recommendations: The EUCAST reference method of MIC determination for MTBC is the broth microdilution method in Middlebrook 7H9-10% OADC medium. The final inoculum is a 105 CFU/mL suspension, obtained from a 10(-2) dilution of a 0.5 McFarland suspension prepared after vortexing bacterial colonies with glass beads before suspending them in sterile water. The culture is maintained in a U-shaped 96well polystyrene microtitre sterile plate with a lid incubated at 36 degrees +/- 1 degrees C. Reading is done using an inverted mirror as soon as the 1:100 diluted control (i.e. 10(3) CFU/mL suspension) shows visual growth. The MIC, expressed in mg/L, is the lowest concentration that inhibits visual growth. Mycobacterium tuberculosis H37Rv ATCC 27294 is used as the reference strain and its targeted MIC values are within the range 0.03-0.12 for isoniazid, 0.12-0.5 for levofloxacin and 0.25-1 mg/L for amikacin. Conclusions: The EUCAST reference method for MTBC was endorsed by EUCAST after public consultation and will from now on be used to define EUCAST ECOFFs and clinical breakpoints. This reference method is not primarily intended to be used under routine conditions and the AST methods will need to be calibrated against this reference method to be used with EUCAST breakpoints. Thomas Sch_on, Clin Microbiol Infect 2020;26:1488 (c) 2020 The Authors. Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- Broth microdilution; EUCAST; MIC; Reference method; Tuberculosis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Schön, Thomas
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Werngren, Jim
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Machado, Diana
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Borroni, Emanuel ...
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Wijkander, Maria
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Lina, Gerard
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Mouton, Johan
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Matuschek, Erika
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Kahlmeter, Gunna ...
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Giske, Christian
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Santin, Miguel
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Cirillo, Daniela ...
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Viveiros, Miguel
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Cambau, Emmanuel ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
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Linköpings universitet
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Karolinska Institutet