Sökning: onr:"swepub:oai:DiVA.org:liu-18549" >
Association Between...
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Elander, NilsLinköpings universitet,Cellbiologi,Hälsouniversitetet
(författare)
Association Between Adenomatosis Polyposis Coli Functional Status and Microsomal Prostaglandin E Synthase-1 Expression in Colorectal Cancer
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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2008-11-26
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Wiley,2009
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-18549
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-18549URI
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https://doi.org/10.1002/mc.20500DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Bioactive metabolites downstream of cyclooxygenase-2 (COX-2) generated prostaglandin H-2 (PGH(2)), in particular prostaglandin E-2 (PGE(2)), are thought to play critical roles during the development of colorectal tumors. Previous reports reveal that defects of the tumor suppressor adenomatosis polyposis coli (APC) contribute to COX-2 upregulation in colon tumor cells. We investigated whether a similar relation was present between APC functional status and the expression of microsomal prostaglandin E synthase-1 (mPGES-1), which acts downstream of COX-2 and catalyses the terminal conversion of PGH(2) into PGE(2). Surprisingly, mPGES-1 mRNA and protein levels were upregulated upon induction of a wild type-APC carrying vector in HT29 colon cancer cells, and downregulated following siRNA silencing of APC in HCT-116 cells. mPGES-1 was overall enhanced in human colorectal tumor specimens versus corresponding non-tumor mucosa and, in accordance with data on HT29 and HCT116 cells, higher levels of mPGES-1 were observed among tumors carrying wild type versus mutant APC. RNAi silencing of beta-catenin and luciferase assays regarding the mPGES-1 promoter region did not reveal a role for APC or beta-catenin/Tcf in controlling mPGES-1 gene transcription. However, RNA degradation assays in HT29 cells revealed a suppressed degradation of mPGES-1 in the presence of wild type APC, implying that mPGES-1 mRNA is stabilized in the APC wild type state. Collectively, we demonstrate a novel association between APC functional status and mPGFS-1 expression in colorectal tumor cells, being most likely related to reduced mPGES-1 mRNA degradation rate in the APC wild type state.
Ämnesord och genrebeteckningar
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cyclooxygenase 2
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Prostaglandin E-2
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beta-catenin
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adenomatosis polyposis coli (APC)
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microsomal prostaglandin E synthase-1 (mPGES-1)
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colorectal cancer
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Zhou, JianlinLinköpings universitet,Institutionen för klinisk och experimentell medicin,Hälsouniversitetet(Swepub:liu)jiazh28
(författare)
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Ungerbäck, JonasLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)jonun74
(författare)
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Dimberg, JanJönköping University
(författare)
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Söderkvist, PeterLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)petso43
(författare)
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Linköpings universitetCellbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Molecular Carcinogenesis: Wiley48:5, s. 401-4070899-19871098-2744
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