Sökning: onr:"swepub:oai:DiVA.org:liu-40129" >
Reshaping the foldi...
-
Grankvist, Hannah,1976-Linköpings universitet,Filosofiska fakulteten,Tema hälsa och samhälle
(författare)
Reshaping the folding energy landscape by chloride salt : Impact on molten-globule formation and aggregation behavior of carbonic anhydrase
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
-
2004-04-22
-
Wiley,2004
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:liu-40129
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-40129URI
-
https://doi.org/10.1016/j.febslet.2004.03.105DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
During chemical denaturation different intermediate states are populated or suppressed due to the nature of the denaturant used. Chemical denaturation by guanidine-HCl (GuHCl) of human carbonic anhydrase II (HCA II) leads to a three-state unfolding process (Cm,NI=1.0 and Cm,IU=1.9 M GuHCl) with formation of an equilibrium molten-globule intermediate that is stable at moderate concentrations of the denaturant (1-2 M) with a maximum at 1.5 M GuHCl. On the contrary, urea denaturation gives rise to an apparent two-state unfolding transition (Cm=4.4 M urea). However, 8-anilino-1-naphthalene sulfonate (ANS) binding and decreased refolding capacity revealed the presence of the molten globule in the middle of the unfolding transition zone, although to a lesser extent than in GuHCl. Cross-linking studies showed the formation of moderate oligomer sized (300 kDa) and large soluble aggregates (>1000 kDa). Inclusion of 1.5 M NaCl to the urea denaturant to mimic the ionic character of GuHCl leads to a three-state unfolding behavior (Cm,NI=3.0 and Cm,IU=6.4 M urea) with a significantly stabilized molten-globule intermediate by the chloride salt. Comparisons between NaCl and LiCl of the impact on the stability of the various states of HCA II in urea showed that the effects followed what could be expected from the Hofmeister series, where Li+ is a chaotropic ion leading to decreased stability of the native state. Salt addition to the completely urea unfolded HCA II also led to an aggregation prone unfolded state, that has not been observed before for carbonic anhydrase. Refolding from this state only provided low recoveries of native enzyme. © 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Ämnesord och genrebeteckningar
-
SOCIAL SCIENCES
-
SAMHÄLLSVETENSKAP
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Borén, KristinaIFM Institutionen för Kemi
(författare)
-
Hammarström, PerIFM Institutionen för Kemi
(författare)
-
Carlsson, UnoIFM Institutionen för Kemi
(författare)
-
Linköpings universitetFilosofiska fakulteten
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:FEBS Letters: Wiley566:1-3, s. 95-990014-57931873-3468
Internetlänk
Hitta via bibliotek
Till lärosätets databas