Sökning: onr:"swepub:oai:DiVA.org:liu-45905" >
The photosystem II-...
The photosystem II-associated Cah3 in Chlamydomonas enhances the O-2 evolution rate by proton removal
-
- Shutova, Tatiana, 1964- (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden
-
- Kenneweg, Hella (författare)
- Univ Osnabruck, Dept Biophys, Osnabruck, Germany
-
- Buchta, Joachim (författare)
- Free Univ Berlin, Dept Phys, D-1000 Berlin, Germany
-
visa fler...
-
- Nikitina, Julia (författare)
- Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142292, Russia
-
- Terentyev, Vasily (författare)
- Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142292, Russia
-
- Chernyshov, Sergey (författare)
- Russian Acad Sci, Branch Shemyakin & Ovchinnikov, Inst Bioorgan Chem, Pushchino 142292, Russia
-
- Andersson, Bertil (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
-
- Allakhverdiev, Suleyman I. (författare)
- Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142292, Russia
-
- Klimov, Vyacheslav V. (författare)
- Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142292, Russia
-
- Dau, Holger (författare)
- Free Univ Berlin, Dept Phys, D-1000 Berlin, Germany
-
- Junge, Wolfgang (författare)
- Univ Osnabruck, Dept Biophys, Osnabruck, Germany
-
- Samuelsson, Göran, 1951- (författare)
- Umeå universitet,Umeå Plant Science Centre (UPSC),Institutionen för fysiologisk botanik,Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden
-
visa färre...
-
(creator_code:org_t)
- 2008-01-31
- 2008
- Engelska.
-
Ingår i: EMBO Journal. - : Wiley. - 0261-4189 .- 1460-2075. ; 27:5, s. 782-791
- Relaterad länk:
-
https://europepmc.or...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Water oxidation in photosystem II ( PSII) is still insufficiently understood and is assumed to involve HCO3-. A Chlamydomonas mutant lacking a carbonic anhydrase associated with the PSII donor side shows impaired O-2 evolution in the absence of HCO3-. The O-2 evolution for saturating, continuous illumination (R-O2) was slower than in the wild type, but was elevated by HCO3- and increased further by Cah3. The R-O2 limitation in the absence of Cah3/HCO3- was amplified by H2O/D2O exchange, but relieved by an amphiphilic proton carrier, suggesting a role of Cah3/HCO3- in proton translocation. Chlorophyll fluorescence indicates a Cah3/HCO3- effect at the donor side of PSII. Time-resolved delayed fluorescence and O-2-release measurements suggest specific effects on proton-release steps but not on electron transfer. We propose that Cah3 promotes proton removal from the Mn complex by locally providing HCO3-, which may function as proton carrier. Without Cah3, proton removal could become rate limiting during O-2 formation and thus, limit water oxidation under high light. Our results underlie the general importance of proton release at the donor side of PSII during water oxidation.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Nyckelord
- carbonic anhydrase
- Chlamydomonas reinhardtii
- photosystem II
- proton removal
- water oxidation
- NATURAL SCIENCES
- NATURVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Shutova, Tatiana ...
-
Kenneweg, Hella
-
Buchta, Joachim
-
Nikitina, Julia
-
Terentyev, Vasil ...
-
Chernyshov, Serg ...
-
visa fler...
-
Andersson, Berti ...
-
Allakhverdiev, S ...
-
Klimov, Vyachesl ...
-
Dau, Holger
-
Junge, Wolfgang
-
Samuelsson, Göra ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biofysik
- Artiklar i publikationen
-
EMBO Journal
- Av lärosätet
-
Linköpings universitet
-
Umeå universitet