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Molecular Fingerpri...
Molecular Fingerprint of Neuropeptide S-Producing Neurons in the Mouse Brain
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- Liu, Xiaobin (författare)
- University of California Irvine
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- Zeng, Joanne (författare)
- University of California Irvine
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- Zhou, Anni (författare)
- University of California Irvine
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- Theodorsson, Elvar (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet
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- Fahrenkrug, Jan (författare)
- University of Copenhagen
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- Reinscheid, Rainer K (författare)
- University of California Irvine
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(creator_code:org_t)
- 2011-05-23
- 2011
- Engelska.
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Ingår i: JOURNAL OF COMPARATIVE NEUROLOGY. - : John Wiley and Sons, Ltd. - 0021-9967. ; 519:10, s. 1847-1866
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Neuropeptide S (NPS) has been associated with a number of complex brain functions, including anxiety-like behaviors, arousal, sleep-wakefulness regulation, drug-seeking behaviors, and learning and memory. In order to better understand how NPS influences these functions in a neuronal network context, it is critical to identify transmitter systems that control NPS release and transmitters that are co-released with NPS. For this purpose, we generated several lines of transgenic mice that express enhanced green-fluorescent protein (EGFP) under control of the endogenous NPS precursor promoter. NPS/EGFP-transgenic mice show anatomically correct and overlapping expression of both NPS and EGFP. A total number of similar to 500 NPS/EGFP-positive neurons are present in the mouse brain, located in the pericoerulear region and the Kolliker-Fuse nucleus. NPS and transgene expression is first detectable around E14, indicating a potential role for NPS in brain development. EGFP-positive cells were harvested by laser-capture microdissection, and mRNA was extracted for expression profiling by using microarray analysis. NPS was found co-localized with galanin in the Kolliker-Fuse nucleus of the lateral parabrachial area. A dense network of orexin/hypocretin neuronal projections contacting pericoerulear NPS-producing neurons was observed by immunostaining. Expression of a distinct repertoire of metabotropic and ionotropic receptor genes was identified in both NPS neuronal clusters that will allow for detailed investigations of incoming neurotransmission, controlling neuronal activity of NPS-producing neurons. Stress-induced functional activation of NPS-producing neurons was detected by staining for the immediate-early gene c-fos, thus supporting earlier findings that NPS might be part of the brain stress response network.
Nyckelord
- transgenic mice
- green-fluorescent protein
- microarray
- gene expression
- locus coeruleus
- Kolliker-Fuse nucleus
- galanin
- development
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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