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- Liu, XiaobinUniversity of California Irvine (författare)
Molecular Fingerprint of Neuropeptide S-Producing Neurons in the Mouse Brain
- Artikel/kapitelEngelska2011
Förlag, utgivningsår, omfång ...
- 2011-05-23
- John Wiley and Sons, Ltd,2011
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:liu-69175
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-69175URI
- https://doi.org/10.1002/cne.22603DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
Klassifikation
Anmärkningar
- Neuropeptide S (NPS) has been associated with a number of complex brain functions, including anxiety-like behaviors, arousal, sleep-wakefulness regulation, drug-seeking behaviors, and learning and memory. In order to better understand how NPS influences these functions in a neuronal network context, it is critical to identify transmitter systems that control NPS release and transmitters that are co-released with NPS. For this purpose, we generated several lines of transgenic mice that express enhanced green-fluorescent protein (EGFP) under control of the endogenous NPS precursor promoter. NPS/EGFP-transgenic mice show anatomically correct and overlapping expression of both NPS and EGFP. A total number of similar to 500 NPS/EGFP-positive neurons are present in the mouse brain, located in the pericoerulear region and the Kolliker-Fuse nucleus. NPS and transgene expression is first detectable around E14, indicating a potential role for NPS in brain development. EGFP-positive cells were harvested by laser-capture microdissection, and mRNA was extracted for expression profiling by using microarray analysis. NPS was found co-localized with galanin in the Kolliker-Fuse nucleus of the lateral parabrachial area. A dense network of orexin/hypocretin neuronal projections contacting pericoerulear NPS-producing neurons was observed by immunostaining. Expression of a distinct repertoire of metabotropic and ionotropic receptor genes was identified in both NPS neuronal clusters that will allow for detailed investigations of incoming neurotransmission, controlling neuronal activity of NPS-producing neurons. Stress-induced functional activation of NPS-producing neurons was detected by staining for the immediate-early gene c-fos, thus supporting earlier findings that NPS might be part of the brain stress response network.
Ämnesord och genrebeteckningar
- transgenic mice
- green-fluorescent protein
- microarray
- gene expression
- locus coeruleus
- Kolliker-Fuse nucleus
- galanin
- development
- MEDICINE
- MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Zeng, JoanneUniversity of California Irvine (författare)
- Zhou, AnniUniversity of California Irvine (författare)
- Theodorsson, ElvarÖstergötlands Läns Landsting,Linköpings universitet,Klinisk kemi,Hälsouniversitetet(Swepub:liu)elvth65 (författare)
- Fahrenkrug, JanUniversity of Copenhagen (författare)
- Reinscheid, Rainer KUniversity of California Irvine (författare)
- University of California IrvineKlinisk kemi (creator_code:org_t)
Sammanhörande titlar
- Ingår i:JOURNAL OF COMPARATIVE NEUROLOGY: John Wiley and Sons, Ltd519:10, s. 1847-18660021-9967
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