Optical manipulation and microfluidics for studies of single cell dynamics

Eriksson, Emma,1980Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Physics Department, Göteborg University (författare)

Validerad; 2007; Bibliografisk uppgift: Special Issue: Optical Manipulation; 20070508 (ramser)
Most research on optical manipulation aims towards investigation and development of the system itself. In this paper we show how optical manipulation, imaging and microfluidics can be combined for investigations of single cells. Microfluidic systems have been fabricated and are used, in combination with optical tweezers, to enable environmental changes for single cells. The environment within the microfluidic system has been modelled to ensure control of the process. Three biological model systems have been studied with different combinations of optical manipulation, imaging techniques and microfluidics. In Saccharomyces cerevisiae, environmentally induced size modulations and spatial localization of proteins have been studied to elucidate various signalling pathways. In a similar manner the oxygenation cycle of single red blood cells was triggered and mapped using Raman spectroscopy. In the third experiment the forces between the endoplasmic reticulum and chloroplasts were studied in Pisum sativum and Arabidopsis thaliana. By combining different techniques we make advanced biological research possible, revealing information on a cellular level that is impossible to obtain with traditional techniques.

Scrimgeour, Jan,1980Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Physics Department, Göteborg University(Swepub:gu)xscrja (författare)
Graneli, Annette,1973Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Physics Department, Göteborg University(Swepub:gu)xgrane (författare)
Ramser, KerstinLuleå tekniska universitet,Signaler och system(Swepub:cth)gu96kera (författare)
Wellander, Rikard,1982Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Physics Department, Göteborg University(Swepub:gu)xwelri (författare)
Enger, Jonas,1966Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Physics Department, Göteborg University(Swepub:gu)xengej (författare)
Hanstorp, Dag,1960Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Physics Department, Göteborg University(Swepub:gu)xhanda (författare)
Goksör, Mattias,1975Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Physics Department, Göteborg University(Swepub:gu)xgokma (författare)
Göteborgs universitetInstitutionen för fysik (GU) (creator_code:org_t)

Ingår i:Journal of Optics. A, Pure and applied optics9:8, s. 113-1211464-42581741-35671361-6617

Av författaren/redakt...: Eriksson, Emma, ...; Scrimgeour, Jan, ...; Graneli, Annette ...; Ramser, Kerstin; Wellander, Rikar ...; Enger, Jonas, 19 ...; visa fler...; Hanstorp, Dag, 1 ...; Goksör, Mattias, ...; visa färre...

Om ämnet

TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Medicinteknik; och Annan medicintek ...

Av lärosätet: Luleå tekniska universitet; Göteborgs universitet; Chalmers tekniska högskola

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