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Induction of morpho...
Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy
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- El-Schich, Zahra (författare)
- Lund University,Lunds universitet,Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Experimentell cancerforskning, Malmö,Forskargrupper vid Lunds universitet,Experimental Cancer Research, Malmö,Lund University Research Groups,Malmö University
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- Mölder, Anna (författare)
- Phase Holographic Imaging AB, Lund,Phase Holographic Imaging AB, Lund
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- Tassidis, Helena (författare)
- Lund University,Lunds universitet,Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine,Man and Biosphere Health (MABH)
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- Härkönen, Pirkko (författare)
- Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine,Finland
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- Falck Miniotis, Maria (författare)
- Lund University,Lunds universitet,Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Experimentell cancerforskning, Malmö,Forskargrupper vid Lunds universitet,Experimental Cancer Research, Malmö,Lund University Research Groups,Malmö University
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- Gjörloff Wingren, Anette (författare)
- Kristianstad University,Lund University,Lunds universitet,Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine,Malmö University
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(creator_code:org_t)
- Elsevier, 2015
- 2015
- Engelska.
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Ingår i: Journal of Structural Biology. - : Elsevier. - 1047-8477 .- 1095-8657. ; 189:3, s. 207-212
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http://dx.doi.org/10...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We are using the label-free technique of holographic microscopy to analyze cellular parameters including cell number, confluence, cellular volume and area directly in the cell culture environment. We show that death-induced cells can be distinguished from untreated counterparts by the use of holographic microscopy, and we demonstrate its capability for cell death assessment. Morphological analysis of two representative cell lines (L929 and DU145) was performed in the culture flasks without any prior cell detachment. The two cell lines were treated with the anti-tumour agent etoposide for one to three days. Measurements by holographic microscopy showed significant differences in average cell number, confluence, volume and area when comparing etoposide-treated with untreated cells. The cell volume of the treated cell lines was initially increased at early time-points. By time, cells decreased in volume, especially when treated with high doses of etoposide. In conclusion, we have shown that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death. Future applications could include real-time monitoring of these holographic microscopy parameters in cells in response to clinically relevant compounds.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- digital holographic microscopy
- volume
- cell death
- viability
- cell morphology
- imaging
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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