Peptide discs as precursors of biologically relevant supported lipid bilayers

Luchini, AlessandraNiels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark (författare)

Supported lipid bilayers (SLBs) are commonly used to investigate the structure and dynamics of biological membranes. Vesicle fusion is a widely exploited method to produce SLBs. However, this process becomes less favoured when the vesicles contain complex lipid mixtures, e.g. natural lipid extracts. In these cases, it is often necessary to change experimental parameters, such as temperature, to unphysiological values to trigger the SLB formation. This may induce lipid degradation and is also not compatible with including membrane proteins or other biomolecules into the bilayers. Here, we show that the peptide discs, ~10 nm discoidal lipid bilayers stabilized in solution by a self-assembled 18A peptide belt, can be used as precursors for SLBs. The characterizations by means of neutron reflectometry and attenuated total reflectance-FTIR spectroscopy show that SLBs were successfully formed both from synthetic lipid mixtures (surface coverage 90-95%) and from natural lipid mixtures (surface coverage ~85%). Traces of 18A peptide (below 0.02 M ratio) left at the support surface after the bilayer formation do not affect the SLB structure. Altogether, we demonstrate that peptide disc formation of SLBs is much faster than the SLB formation by vesicle fusion and without the need of altering any experimental variable from physiologically relevant values.

Sebastiani, FedericaMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ai3677 (författare)
Tidemand, Frederik GrønbækNiels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark (författare)
Batchu, Krishna ChaithanyaInstitut Laue Langevin, 71 avenue des Martyrs, 38000 Grenoble, France (författare)
Campana, MarioISIS-STFC, Rutherford Appleton Laboratory, Chilton, Oxon OX11 0QX, United Kingdom (författare)
Fragneto, GiovannaInstitut Laue Langevin, 71 avenue des Martyrs, 38000 Grenoble, France (författare)
Cárdenas, MaritéMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ae0614 (författare)
Arleth, LiseNiels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark (författare)
Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, DenmarkInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Ingår i:Journal of Colloid and Interface Science: Elsevier585, s. 376-3850021-97971095-7103

Av författaren/redakt...: Luchini, Alessan ...; Sebastiani, Fede ...; Tidemand, Freder ...; Batchu, Krishna ...; Campana, Mario; Fragneto, Giovan ...; visa fler...; Cárdenas, Marité; Arleth, Lise; visa färre...

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