Affinity Capillary Electrophoresis as a Tool To Characterize Molecularly Imprinted Nanogels in Solution

Contardi, CeciliaDepartment of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy (författare)

In this work, an innovative and accurate affinity capillary electrophoresis (ACE) method was set up to monitor the complexation of aqueous MIP nanogels (NGs) with model cancer-related antigens. Using α2,6′- and α2,3′-sialyllactose as oversimplified cancer biomarker-mimicking templates, NGs were synthesized and characterized in terms of size, polydispersity, and overall charge. A stability study was also carried out in order to select the best storage conditions and to ensure product quality. After optimization of capillary electrophoresis conditions, injection of MIP NGs resulted in a single, sharp, and efficient peak. The mobility shift approach was applied to quantitatively estimate binding affinity, in this case resulting in an association constant of K ≈ 106 M–1. The optimized polymers further displayed a pronounced discrimination between the two sialylated sugars. The newly developed ACE protocol has the potential to become a very effective method for nonconstrained affinity screening of NG in solution, especially during the NG development phase and/or for a final accurate quantitation of the observed binding.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
polymer nanoparticles
sialic-acid
expression
beta(2)-microglobulin
glycosylation
adsorption
isotherm
gold

Rubes, DavideDepartment of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy (författare)
Serra, MassimoDepartment of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy (författare)
Dorati, RossellaDepartment of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy (författare)
Dattilo, MarcoDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Arcavacata di Rende, CS, Italy (författare)
Mavliutova, LiliiaMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ah6529 (författare)
Patrini, MaddalenaDepartment of Physics, University of Pavia, 27100 Pavia, PV, Italy (författare)
Guglielmann, RaffaellaDepartment of Mathematics F. Casorati, University of Pavia, 27100 Pavia, PV, Italy (författare)
Sellergren, BörjeMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ab8456 (författare)
De Lorenzi, ErsiliaDepartment of Drug Sciences, University of Pavia, 27100 Pavia, PV, Italy (författare)
Department of Drug Sciences, University of Pavia, 27100 Pavia, PV, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Arcavacata di Rende, CS, Italy (creator_code:org_t)

Ingår i:Analytical Chemistry: American Chemical Society (ACS)96:7, s. 3017-30240003-27001520-6882

Av författaren/redakt...: Contardi, Cecili ...; Rubes, Davide; Serra, Massimo; Dorati, Rossella; Dattilo, Marco; Mavliutova, Lili ...; visa fler...; Patrini, Maddale ...; Guglielmann, Raf ...; Sellergren, Börj ...; De Lorenzi, Ersi ...; visa färre...

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