Sökning: onr:"swepub:oai:DiVA.org:su-142613" >
Expression of a Dro...
-
Tzafestas, Kyriakos
(författare)
Expression of a Drosophila glutathione transferase in Arabidopsis confers the ability to detoxify the environmental pollutant, and explosive, 2,4,6-trinitrotoluene
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
-
2016-12-06
-
Wiley,2017
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:su-142613
-
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-142613URI
-
https://doi.org/10.1111/nph.14326DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
The explosive 2,4,6-trinitrotoluene (TNT) is a significant, global environmental pollutant that is both toxic and recalcitrant to degradation. Given the sheer scale and inaccessible nature of contaminated areas, phytoremediation may be a viable clean-up approach. Here, we have characterized a Drosophila melanogaster glutathione transferase (DmGSTE6) which has activity towards TNT. Recombinantly expressed, purified DmGSTE6 produces predominantly 2-glutathionyl-4, 6-dinitrotoluene, and has a 2.5-fold higher Maximal Velocity (Vmax), and five-fold lower Michaelis Constant (Km) than previously characterized TNT-active Arabidopsis thaliana (Arabidopsis) GSTs. Expression of DmGSTE6 in Arabidopsis conferred enhanced resistance to TNT, and increased the ability to remove TNT from contaminated soil relative to wild-type plants. Arabidopsis lines overexpressing TNT-active GSTs AtGST-U24 and AtGST-U25 were compromised in biomass production when grown in the absence of TNT. This yield drag was not observed in the DmGSTE6-expressing Arabidopsis lines. We hypothesize that increased levels of endogenous TNT-active GSTs catalyse excessive glutathionylation of endogenous substrates, depleting glutathione pools, an activity that DmGST may lack. In conclusion, DmGSTE6 has activity towards TNT, producing a compound with potential for further biodegradation. Selecting or manipulating plants to confer DmGSTE6-like activity could contribute towards development of phytoremediation strategies to clean up TNT from polluted military sites.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Razalan, Maria M.
(författare)
-
Gyulev, Ivan
(författare)
-
Mazari, Aslam M. A.Stockholms universitet,Institutionen för neurokemi
(författare)
-
Mannervik, BengtStockholms universitet,Institutionen för neurokemi(Swepub:su)bmann
(författare)
-
Rylott, Elizabeth L.
(författare)
-
Bruce, Neil C.
(författare)
-
Stockholms universitetInstitutionen för neurokemi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:New Phytologist: Wiley214:1, s. 294-3030028-646X1469-8137
Internetlänk
Hitta via bibliotek
Till lärosätets databas