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Yoctomole electroch...
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Carinelli, S.Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra, Spain.
(författare)
Yoctomole electrochemical genosensing of Ebola virus cDNA by rolling circle and circle to circle amplification
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
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Elsevier BV,2017
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Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:su-143424
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-143424URI
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https://doi.org/10.1016/j.bios.2016.09.099DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-322442URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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This work addresses the design of an Ebola diagnostic test involving a simple, rapid, specific and highly sensitive procedure based on isothermal amplification on magnetic particles with electrochemical readout. Ebola padlock probes were designed to detect a specific L-gene sequence present in the five most common Ebola species. Ebola cDNA was amplified by rolling circle amplification (RCA) on magnetic particles. Further re-amplification was performed by circle-to-circle amplification (C2CA) and the products were detected in a double-tagging approach using a biotinylated capture probe for immobilization on magnetic particles and a readout probe for electrochemical detection by square-wave voltammetry on commercial screen-printed electrodes. The electrochemical genosensor was able to detect as low as 200 ymol, corresponding to 120 cDNA molecules of L-gene Ebola virus with a limit of detection of 33 cDNA molecules. The isothermal double-amplification procedure by C2CA combined with the electrochemical readout and the magnetic actuation enables the high sensitivity, resulting in a rapid, inexpensive, robust and user-friendly sensing strategy that offers a promising approach for the primary care in low resource settings, especially in less developed countries.
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Kühnemund, MalteUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi(Swepub:uu)malku277
(författare)
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Nilsson, MatsUppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Box 1031, SE-17I21 Solna, Sweden.(Swepub:uu)matsnils
(författare)
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Pividori, M. I.Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra, Spain.
(författare)
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Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra, Spain.Science for Life Laboratory, SciLifeLab
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biosensors & bioelectronics: Elsevier BV93, s. 65-710956-56631873-4235
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