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Acrylamide alters C...
Acrylamide alters CREB and retinoic acid signalling pathways during differentiation of the human neuroblastoma SH-SY5Y cell line
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- Attoff, Kristina (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.
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- Johansson, Ylva (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.
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- Cediel-Ulloa, Andrea (författare)
- Uppsala universitet,Miljötoxikologi,Karolinska Inst, Unit Toxicol Sci, Swedish Toxicol Sci Res Ctr Swetox, Sodertalje, Sweden
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- Lundqvist, Jessica (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Karolinska Institutet, Sweden,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden.;Karolinska Inst, Inst Environm Med, Stockholm, Sweden.
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- Gupta, Rajinder (författare)
- Maastricht Univ, Sch Oncol & Dev Biol GROW, Dept Toxicogen, Maastricht, Netherlands.
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- Caiment, Florian (författare)
- Maastricht Univ, Sch Oncol & Dev Biol GROW, Dept Toxicogen, Maastricht, Netherlands.
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- Gliga, Anda (författare)
- Karolinska Institutet
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- Forsby, Anna (författare)
- Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Uppsala University, Sweden,Institutionen för organismbiologi,Stockholm Univ, Dept Biochem & Biophys, Stockholm, Sweden
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(creator_code:org_t)
- 2020-10-07
- 2020
- Engelska.
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Acrylamide (ACR) is a known neurotoxicant which crosses the blood-brain barrier, passes the placenta and has been detected in breast milk. Hence, early-life exposure to ACR could lead to developmental neurotoxicity. The aim of this study was to elucidate if non-cytotoxic concentrations of ACR alter neuronal differentiation by studying gene expression of markers significant for neurodevelopment in the human neuroblastoma SH-SY5Y cell model. Firstly, by using RNASeq we identified two relevant pathways that are activated during 9 days of retinoic acid (RA) induced differentiation i.e. RA receptor (RAR) activation and the cAMP response element-binding protein (CREB) signalling pathways. Next, by qPCR we showed that 1 and 70 mu M ACR after 9 days exposure alter the expression of 13 out of 36 genes in the RAR activation pathway and 18 out of 47 in the CREB signalling pathway. Furthermore, the expression of established neuronal markers i.e. BDNF, STXBP2, STX3, TGFB1 and CHAT were down-regulated. Decreased protein expression of BDNF and altered ratio of phosphorylated CREB to total CREB were confirmed by western blot. Our results reveal that micromolar concentrations of ACR sustain proliferation, decrease neurite outgrowth and interfere with signalling pathways involved in neuronal differentiation in the SH-SY5Y cell model.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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