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Sökning: onr:"swepub:oai:DiVA.org:su-200948" > Action spectroscopy...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00002954naa a2200397 4500
001oai:DiVA.org:su-200948
003SwePub
008220124s2021 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-2009482 URI
0247 a https://doi.org/10.1063/5.00632582 DOI
040 a (SwePub)su
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Coughlan, Neville J. A.4 aut
2451 0a Action spectroscopy of the isolated red Kaede fluorescent protein chromophore
264 1b AIP Publishing,c 2021
338 a print2 rdacarrier
520 a Incorporation of fluorescent proteins into biochemical systems has revolutionized the field of bioimaging. In a bottom-up approach, understanding the photophysics of fluorescent proteins requires detailed investigations of the light-absorbing chromophore, which can be achieved by studying the chromophore in isolation. This paper reports a photodissociation action spectroscopy study on the deprotonated anion of the red Kaede fluorescent protein chromophore, demonstrating that at least three isomers–assigned to deprotomers–are generated in the gas phase. Deprotomer-selected action spectra are recorded over the S1 ← S0 band using an instrument with differential mobility spectrometry coupled with photodissociation spectroscopy. The spectrum for the principal phenoxide deprotomer spans the 480–660 nm range with a maximum response at ≈610 nm. The imidazolate deprotomer has a blue-shifted action spectrum with a maximum response at ≈545 nm. The action spectra are consistent with excited state coupled-cluster calculations of excitation wavelengths for the deprotomers. A third gas-phase species with a distinct action spectrum is tentatively assigned to an imidazole tautomer of the principal phenoxide deprotomer. This study highlights the need for isomer-selective methods when studying the photophysics of biochromophores possessing several deprotonation sites.
650 7a NATURVETENSKAPx Fysik0 (SwePub)1032 hsv//swe
650 7a NATURAL SCIENCESx Physical Sciences0 (SwePub)1032 hsv//eng
7001 a Stockett, Mark H.u Stockholms universitet,Fysikum4 aut0 (Swepub:su)mstoc
7001 a Kjær, Christina4 aut
7001 a Ashworth, Eleanor K.4 aut
7001 a Bulman Page, Philip C.4 aut
7001 a Meech, Stephen R.4 aut
7001 a Brøndsted Nielsen, Steen4 aut
7001 a Blancafort, Lluís4 aut
7001 a Hopkins, W. Scott4 aut
7001 a Bull, James N.4 aut
7102 a Stockholms universitetb Fysikum4 org
7730 t Journal of Chemical Physicsd : AIP Publishingg 155:12q 155:12x 0021-9606x 1089-7690
8564 u https://ueaeprints.uea.ac.uk/id/eprint/81509/1/Accepted_Manuscript.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-200948
8564 8u https://doi.org/10.1063/5.0063258

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